UOG study to examine genetic connectivity of fish, snails, and shrimp native to Guam and Marianas rivers

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In an effort to manage and conserve diadromous fish, snails, and shrimp that are native to rivers in Guam and the Marianas, a researcher from the University of Guam will be working over the next four years to collect and genetically analyze species found in the region’s watersheds.

Diadromous animals are those that transition between freshwater and saltwater environments at different stages of their life cycles.

Daniel Lindstrom

“Effective conservation management of these aquatic communities starts with discerning their historical genetic connections and/or isolation,” said Associate Professor Daniel Lindstrom, who holds a doctorate in zoology and is overseeing the project.

The work is being funded by the university’s National Science Foundation EPSCoR grant. 

Nature of diadromous animals

Many of the fish, snail, and shrimp species that live in the streams of Southern Guam are spawned in freshwater before drifting into the ocean as larvae before migrating back to freshwater to grow into adults and spawn.

“When the eggs of these species hatch, they get washed out into the ocean,” Lindstrom said. “There is a possibility that the animals you find in freshwater in the island region may all be connected because of this larval marine phase.”

‘Rewriting the book’ on these organisms

Lindstrom plans to collect specimens from each of Southern Guam’s 14 main watersheds and then eventually expand to Saipan and Rota to augment the collection. An initial collection has already been conducted in the Asmafines and Sella Rivers.

As the specimens are collected, they will be photographed, dissected, and then undergo DNA extraction.

“By looking at the genetics of these animals, I can check how similar they are to the same species found in another river on Guam and check the genetic similarity to see the patterns in their population,” Lindstrom said. “Even though those two rivers flow into the ocean less than 50 meters apart, they have different species in them. That’s really strange and I hope our genetic and survey work will find answers for that.”

He is targeting approximately 60 species that have not been genetically confirmed as existing or distinct species and have been referred to with tentative names or listed only by genus but without a species name.

“There’s an ancient Chinese proverb that goes: ‘The first step on the path to true knowledge is getting the names of things right,’ so I’m really excited about that, and I’m hoping that’ll be my scientific legacy on Guam,” he said. “We’re pretty much rewriting the book on the island’s native diadromous organisms.”

Potential to discover new species

It’s possible the research team — including EPSCoR-sponsored graduate students Khanh Ly and Karina Mejia and undergraduate student Louise Pascua — may uncover a few new species along the way.

Khanh Ly

“I’m excited to see what species we can describe and to find new species – whether they’re here or on other islands,” Ly said. “I’d like to publish research about them for other people to use.”

This project will contribute to 30 years of collecting and genetically analyzing specimens from watersheds all over the tropics, including Guam, Saipan, Rota, Hawaii, Japan, the Philippines, Indonesia, Malaysia, Thailand, Panama, Costa Rica, and Nicaragua. 

All of the preserved specimens and tissues collected over the course of the project will go to the GECCO Biorepository, a physical and cyber warehouse of records and images operated by the Guam NSF EPSCoR program. The biorepository can be accessed online at https://specifyportal.uog.edu/.

Two GECCO summer math students accepted to graduate school

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Two University of Guam alumni who participated in the first ever GECCO Summer Math Research Experience (SMRE) have been accepted into grad school!
 
The GECCO Summer Math Research Experience was held from June 7 to July 23, 2021. Participants addressed issues such as overfishing parrotfish, coral bleaching, and coral diseases using mathematical modeling.
 
Henry Song, a recent computer science graduate, says that the Summer Math Research Experience equipped him with the tools and skills to pursue a graduate education.
 
“I have considered applying to grad programs prior to the program, but it was through the advice and guidance of the faculty mentors, research assistants, and overall experience that convinced me to apply to my target school: Seattle University. I hope to follow my dream of becoming a software engineer, and potentially pursue a Ph.D. in the future!” said Song.
 
Song will attend Seattle University this month.
Regina Mae Dominguez, one of two research assistants who served as mentors for the SMRE students, will attend the University of Washington for graduate school.
 
Students wanting to participate in the next Summer Math Research Experience can email epscor.smrp@triton.uog.edu or visit our website for more information.
 

S/T committee explores pandemic recovery opportunities to move priorities forward

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Science Tech Comm 2

At the University of Guam EPSCoR Science and Technology (S/T) Committee meeting earlier this month, members looked at addressing its priority challenge areas by tapping into resources that recently opened to support the recovery of communities during the pandemic. 

The S/T committee’s priority challenge areas include IT and cybersecurity, biosciences and technology transfer, medical and healthcare, and sustainability (food-waste-energy nexus).  

Robert Underwood, committee vice-chair and voting member, said possible funding sources include the Department of Commerce’s Economic Development Administration grants. The EDA grant received around $3 billion from the American Rescue Plan Act to reinvest in communities across the US. The program can support both the development of pandemic recovery strategies and the implementation of recovery projects.  

The recovery projects under the grant are aligned with the focus of the S/T committee. The list of eligible projects includes construction and development of technology-based facilities, wet labs, science and research parks, workforce training facilities, and telecommunications infrastructure. The EDA grant could potentially generate anywhere between $500 thousand to over $5 million in funding.  

With the committee’s mandate to expand the University’s research capabilities, tapping into statewide planning, research, and network grants supported by EDA was also discussed during the meeting.  

The meeting also highlighted several gains achieved by committee members which address the priority challenge areas. For example, Melanie Mendiola, GEDA administrator and committee voting member, provided updates on the government’s plan to build a medical campus facility, which addresses the medical and healthcare challenge area. According to Mendiola, the first phase of the project includes constructing a Center for Disease Control laboratory on Guam. She said the Office of Island Affairs (OIA) under the CDC has approved around $30 million for the project.  

At the start of the meeting, UOG President Thomas Krise also provided updates on the University’s infrastructure projects such as the School Engineering, the Student Center, and the Guam Cultural Repository building. He anticipates the repository to open by the end of this year. Krise also highlighted the importance of delivering the message that research advances can help the community, and activities relating to science and technology are investments that yield returns. 

For the next few months, the S/T steering committee will be developing a new Guam Science and Technology plan focusing on the following areas: Micronesia/international research collaboratorium; STEM capacity-building; STEM infrastructure; communication, and; diversified economy. 

University of Guam biology student wins prize for diatom discoveries in Micronesia

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Britney Sison, an undergraduate biology and chemistry student at the University of Guam, has won an award for her discovery of five potentially undocumented diatoms in mud samples from the Micronesian Islands of Palau, Yap, Pohnpei, and the Marshall Islands. Her discoveries are in addition to two potentially new diatom species found earlier this year by UOG student Gabriella Prelosky.

 

Diatoms are single-celled algae found in oceans, lakes, and rivers. They are primary producers in the food chain as photosynthetic organisms, according to UOG Professor Emeritus of Biology Christopher Lobban, who mentors the student researchers in the Microscopy Teaching & Research Laboratory on the UOG campus.

Sison’s discovery happened during her research fellowship under the NSF INCLUDES: SEAS Islands Alliance program, a $10 million initiative funded by the National Science Foundation to broaden participation in STEM fields of students in U.S. territories and affiliated islands.

 

Her presentation — “New species of conopeate Nitzschia in the Pacific Islands” — won third place in the student poster competition at the 26th International Diatom Symposium, held virtually from Aug. 23 to 25 out of Yamagata, Japan. The symposium brought together hundreds of diatom researchers from around the world.

 

The species Sison focused on in her presentation were just five of many potentially new species of diatoms she found while examining microbial mat and mud samples.

 

Diatom samples from Palau, Yap, Pohnpei, and the Marshall Islands were collected and mailed to the UOG lab by interns in the 2021 NSF INCLUDES Bridge-to-Bachelor’s summer program: Kebang Ngiraklang, a student at the Palau Community College under the mentorship of Vernice Yuki, and Iverson Aliven and Marlin Lee Ling, from the College of Micronesia-FSM under the mentorship of Brian Lynch.

Using the laboratory’s new Scanning Electron Microscope (SEM), which was funded by the university’s Guam NSF EPSCoR grant, Sison and Lobban were able to examine the samples intensively.

 

Sison is now in the process of naming the potentially new species. One of them will be named Nitzschia biseriata because of its unique characteristic of having double rows of pores on its body instead of one.

“‘Two rows’ translated into Latin is ‘biseriate,’” Lobban said. “If there’s an obvious characteristic like that, it’s useful to name it that way because then it will tell people something about the species.”

Sison’s findings will officially be new species once a paper about the diatoms has been peer-reviewed and approved for publication.

“It’s been really interesting to study diatoms,” Sison said. “In the words of Dr. Lobban, it’s like an adult treasure hunt. You never know what you’ll find.”

Both Sison and Prelosky will present more complete results of her project at the 2021 National Diversity in STEM Digital Conference, which will be held by SACNAS from Oct. 25–29. The NSF INCLUDES: SEAS Islands Alliance is administered by the UOG Center for Island Sustainability and Sea Grant programs in partnership with the School of Education at the University of Guam. UOG faculty members Austin Shelton, Cheryl Sangueza, and Else Demeulenaere serve as investigators of the grant award. NSF INCLUDES collaborates closely with the Guam NSF EPSCoR program, also funded by the National Science Foundation.

Diatom herbarium upgrades archival equipment for long-term accessibility

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The diatom herbarium, which is part of the University of Guam (UOG) Herbarium and the Guam Ecosystems Collaboratorium for Corals and Oceans (GECCO) Biorepository is getting new, archival labels in recognition of its permanent value as a repository of diatom samples from the Marianas. The GECCO Biorepository is a physical and cyber warehouse of records and images that is operated by the Guam NSF EPSCoR program, which is funded by the National Science Foundation.

Diatoms are microscopic single-celled algae found in oceans, lakes, and rivers.

“Diatoms produce two-fifths of the oxygen we breathe and are used as water quality indicators in freshwater studies,” said UOG Professor Emeritus of Biology Christopher Lobban. “But for the marine species to be useful as indicators, we first have to find out what species live here and under what conditions.” The collection is a legacy project that was started by Lobban in 1988 and includes samples collected in Guam, Yap, Chuuk, Pohnpei, Palau, and the Marshall Islands. The collection was paused for nearly two decades, but was rekindled in 2007 when new equipment, collaborators, and online access to old literature became available.

As the project grew, it became apparent that it needed to be curated as a collection for long-term accessibility. Once the self-sticking slide labels from its start in 1988 began to fall off from age, Lobban found best practices for museums and acquired archival paper, special adhesives, and custom templates to catalog 3,000 existing slides and label new slides.

“If the slides are to be useful in the future the labels need to stay on,” said Lobban. “The label indicates the sample number, which refers to the collecting information in the lab notebooks and database. Not knowing where and when the samples were collected significantly decreases their scientific value.” There are over 3,300 slides and 1,600 scanning electron microscope stubs, along with raw materials and remainders in the diatom herbarium, which is located in the Microscopy Teaching and Research Lab.

The list of cataloged materials has been entered into the Guam NSF EPSCoR Biorepository online database. A long-term project is underway to get all of the imaged specimens added along with their images. The online database can be accessed at https://specifyportal.uog.edu.

Students in GECCO’s first summer math program study overfishing, coral disease

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The effects of overfishing parrotfish, coral bleaching, and coral diseases are some of the local issues that students sought to address using mathematical modeling this summer. The students — five undergraduate students and one recent graduate — were part of the first-ever Summer Math Research Experience held from June 7 to July 23 at the University of Guam.

“The more I dove into modeling systems in this program, I’ve realized that math can actually do a lot,” said Henry Song, a recent computer science graduate. “I didn’t realize that something like coral reefs were able to be modeled by math. It was a really good learning experience.” 

The summer program was part of the Guam Ecosystems Collaboratorium for Corals & Oceans (GECCO) project, funded by the NSF EPSCoR grant. Part of the project’s aim is to increase the number and diversity of students who pursue careers in STEM. 

“All of the projects this year were focused on local issues, so I was excited to help out this summer,” said Regina Mae Dominguez, one of two research assistants who served as mentors for the students.

The Summer Math Research Experience was held in conjunction with two other research experience programs: the Young Research Experience in Mathematics and the National Research Experience for Undergraduates Program. Students in these programs investigated the effects of pathogens on the population dynamics of the coconut rhinoceros beetle while the other group developed models to study the population dynamics of the Mariana eight-spot butterfly and parasitoid wasps. 

The students’ work from this first GECCO summer program will assist modeling efforts in the Common Garden Project, a four-year EPSCoR-funded study launched in August that will examine three habitat-forming coral species over a multi-year span and their responses to environmental change. 

“By Year 2, we should have a dataset ready,” said Dr. Bastian Bentlage, associate professor of bioinformatics. “Halfway through this year’s math program, we collected the first two data points for the Common Garden. Since we’re interested in seasonal dynamics, having data from a full season should allow them to apply that data to their models.”

Once the models have been developed, they will be used to support reef management and intervention strategies. 

“We’re going to model disease transmission and the corals’ responses to environmental stress,” said Dr. Leslie Aquino, associate professor of mathematics. “The math faculty will look at what the students this year have done, and our next set will continue to build on these models or expand on them.” 

Participate in the Summer Math Research Experience

Students wanting to participate in the next Summer Math Research Experience can email epscor.smrp@triton.uog.edu or visit https://guamepscor.uog.edu/smre/ for more information. 

G3 Conservation Corps helps plant more than 2,000 trees to prevent erosion

G3 Group tree planting
G3 Group tree planting

The growing reforestation movement was in full display as more than 2,000 trees were planted in the hills of Malesso’ on July 24 by the Guam Green Growth Conservation Corps, a joint program of the University of Guam Center for Island Sustainability, the Office of the Governor, and Guam NSF EPSCoR’s Education and Workforce Development objective.

The Corps worked alongside the Department of Agriculture Forestry Division, and more than 200 interns and volunteers.

The effort was part of the As Gadao Manell Watershed Restoration Project and one of several Island Beautification Task Force activities organized by the Office of the Lieutenant Governor of Guam to commemorate Guam’s 77th Liberation Day. 

“[The Forestry Division] is trying to plant trees to prevent erosion, to put back and recover native and endemic species, and try and solve some environmental issues out there,” Lt. Gov. Joshua Tenorio said. “Tree planting this morning is one of the very critical pieces in the plan.”

The G3 Conservation Corps members spent the week prior working alongside the DOA team preparing the land for the planting event.

“We spent about four days bush-cutting in a very specific manner, taught by the forestry team, to prepare the land for this event, and it made it so easy for the volunteers to come in and plant so many trees,” said G3 Conservation Corps member Joey Certeza.  “Preparing the land, and now seeing it with so many people helping out, is very satisfying and gratifying.” 

The corps members created contour lines in the southern ridge to create space for trees to be planted and pruned previously planted acacia trees (Acacia auriculiformis) to prepare for the introduction of understory natives to develop a native forest.

“We are working to convert the highly fire-prone landscape, which is dominated by invasive grass species, to forested lands,” said Christine Fejeran, the Forestry and Soil Resources Division chief at the Guam Department of Agriculture. “The acacias help amend soils and shade out the grasses giving native trees a chance, once planted.”

The G3 Conservation Corps is a newly launched workforce development program preparing the local community for the emerging green economy. It is part of the Guam Green Growth initiative, the island’s most comprehensive public-private partnership ever created to achieve a sustainable future for the island. 

UOG Marine Lab students cleanup the beach

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Students and researchers from the UOG Marine Laboratory came out last Saturday morning to clean up a part of Pago Bay.

The beach cleanup was organized by Anela Duenas, an NSF INCLUDES: SEAS Islands Alliance research fellow who is being mentored by Dr. Bastian Bentlage.

“I really liked the idea of cleaning up the beach that is connected to the bay where we take our seawater from to use for our research. It helped me feel more connected to what we do and think about how we can help more beyond the research that we do at the marine lab,” Duenas said.

Flyers were posted last week throughout the UOG Marine Laboratory to invite people to join the event. Participants found items such as fishing lines, bottles, wrappers, a grill, and a hat.

Study will identify traits that make coral species resilient to climate change

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As the planet experiences heatwaves, warming seas, and other effects of climate change, researchers from the University of Guam (UOG) will examine how these impacts may affect the structure of the island’s coral reefs by identifying species-specific responses to environmental change.

The experiment, which is being funded by the university’s National Science Foundation EPSCoR grant, will study three habitat-forming coral species that are dominant throughout the island’s shallow reef flats, two of which exist in distinctly different color morphologies. These species include: the boulder-like massive Porites, which grows in both brown and purple morphologies; the fingerlike Porites cylindrica, which grows in brown and yellow morphologies; and the staghorn coral Acropora cf. pulchra. Cuttings of each coral species and color morph were planted in four plots in two sections of the Piti Marine Preserve and will be monitored for the next four years.

“We hope to learn about which of these species and their color morphs have characteristics that may confer better resilience to climate change,” said Professor Laurie Raymundo, the interim director of the UOG Marine Laboratory. “That will allow us to examine why they’re doing better and why some of them aren’t doing as well. Eventually, we may be able to make some predictions about how Guam’s reefs may change in the future.”

This project is unique because many studies are conducted within a much shorter time period.

“In most cases, people conduct an experiment for a whole season,” said UOG Associate Professor Bastian Bentlage. “There are not a lot of datasets – especially over

a multi-year span – that look at individual corals and really provide data on how they get stressed, and recover, and follow the long-term effects of experiencing that stress.”

The plots will be checked biweekly during the bleaching season from July to October and monthly from November to June, when sea surface temperatures are cooler.

The data collected from this experiment will be used by a team of mathematics professors and students at the university to model disease transmission and responses to stress, to better inform reef management and intervention strategies.

“We hope to gain a better understanding of what Guam’s reefs will look like in the future and what kind of traits lend themselves to coral resilience so that we can implement control measures that will result in a healthier coral reef ecosystem,” said UOG Associate Professor Leslie Aquino. “We’re really seeing the benefits of this cross collaboration between the Math and Marine Laboratory teams and sparking new ideas and better understanding for both groups of how these models and coral reef ecosystems work.”

New reefs

At the end of the experiment, each of the plots will be left to grow into new reef assemblages as part of a permit agreement with the Guam Department of Agriculture. Monitoring beyond this project may continue to contribute to an even longer-term data set that can continue to inform management.

During the initial planting of the coral cuttings, Bentlage noticed that young fish were attracted to and visiting the plots. According to a study based in Fiji, juvenile fish are able to smell the difference between good and bad reefs.

“I still think that was one of the coolest, most eye-opening things,” said Bentlage. “It was really interesting to see how it attracted the fish community that now seems to be resident in these plots. I hope that I can come back five to ten years from now and see how our experimental plot turned into the seedling of a new reef track.”

Committee begins development on STEM capacity plan

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Science and Technology Meeting 1

Members of the Guam Science and Technology Steering Committee met on June 29 to discuss ways to bolster the island’s STEM capacity and improve connectivity throughout the Micronesia and the region. 

Comprised of 17 members from government, business, and academic communities, the committee oversees the Guam EPSCoR program’s Guam Ecosystems Collaboratorium for Corals and Oceans (GECCO). The committee also oversees the Guam NASA EPSCoR program.   

Over the next three to six months, the committee will develop a five-year plan that will focus on achieving five objectives:  

  • Serve as a state-of-the-art regional research hub for Guam and the region  
  • Build STEM capacity through education and workforce development  
  • Support STEM infrastructure and identify opportunities in the public sector for potential economic activity  
  • Promote increased communication about STEM throughout Micronesia and the region for informed decision making, knowledge sharing, and continuing education  
  • Create a diversified economy  

“The plan is in development, but its purpose is to blend research knowledge and private sector, economic development, and government investment and effort into something that benefits our society,” said Dr. Robert Underwood, President Emeritus of the University of Guam, who serves as the committee’s vice chair. “First, we’re going to have to have some consultation from economic players – people who would inform us about what plans and activities they have in mind so that we’re informed as a committee.”  

Melanie Mendiola, the chief executive officer of the Guam Economic Development Authority, and Docomo Pacific President Roderick Boss were elected to serve as the committee’s co-chairs.  

Gary Hiles, the chief economist of the Guam Department of Labor, presented information gathered from census data about the categories of STEM employment possibilities and jobs available on Guam. During his presentation, Hiles noted that registered nurses, civil engineers, and computer specialists are the most common STEM occupations on the island.  

“Next time, we may hear from someone about the work they’re doing economically or for the military buildup,” said Underwood. “We may want the governor to talk about her vision, or the speaker to get the legislature’s opinion, or someone from the chamber of commerce. In my own mind, I’m trying to figure out how to best secure that input and see how we can benefit from that.” 

After Hiles’ presentation, the committee members discussed potential areas of economic and research activities to prioritize such as solid waste issues, application of marine science research, creating a teaching hospital for medical professionals, and cybersecurity development.  

At the end of the meeting, the committee members were tasked to indicate which two out of the five objectives they would like to work on. Teams will be organized around the objectives and additional members may be drawn from external sources in the private sector, government, and educational institutions.  

The board will meet on a bimonthly basis. The next anticipated meeting will be in August. Guam EPSCoR is funded by the National Science Foundation (NSF).  

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