Guam NSF EPSCoR supports sustainable fisheries through fish maturity workshop

Jungle Histology Photo 1

From May 28 to June 14, 2024, graduate students from the University of Guam as well as scientists from Saipan attended a workshop called Jungle Histology: Fish Gonad Analysis at the UOG Marine Laboratory to learn how to determine the sex and maturity of reef fishes.

This workshop was the final segment of the two-part, weeklong Fish Life History Workshop series funded by Guam NSF EPSCoR to enhance regional research capacity within Micronesia and improve the management of fisheries within the Pacific.

Three visiting scientists from Hawai’i facilitated the session: Ken Longenecker, Ph.D., an associate researcher at Bishop Museum; Ross Langston, Ph.D., a professor of biology at Windward Community College; and Erik Franklin, Ph.D., an associate research professor at the Hawai’i Institute of Marine Biology at the University of Hawai’i at Manoa.

According to Longenecker, knowing whether a fish is mature is essential to the sustainability of fisheries.

“At the most basic level, one of the things that people seem to understand is that it’s a good idea not to catch a fish and eat it until it’s had the chance to reproduce,” said Longenecker. “But we don’t know the size at which fish reach maturity for about 83 percent of exploited species worldwide.”

Histology is a discipline of biology that examines the microscopic anatomy of biological tissues. In the case of fish histology, the gonads of a specimen are extracted, processed, and then examined with a microscope.

Typically, histological practices require working in a laboratory with specialized equipment, but according to Langston, the method of jungle histology is unique because its needs are minimal.

“We can do this work with just a few batteries, so we don’t need much in the way of infrastructure,” said Langston.

Bolstering regional research capacity
Workshops like this one are crucial in supporting communities within the Pacific who rely on their fisheries for sustenance.

“For a lot of places like Saipan and Guam and other areas in the Pacific, fish are the primary or even the only source of protein that some people have in their diets, so it’s extremely important that we are able to give them data so that they can confidently manage their harvest year after year without depleting their stocks,” said Franklin.

Improving regional research capacity allows scientists to answer questions so that they can serve the interests of their communities and even teach other researchers skills that they have learned.

“There are resources here I don’t have back home, so I look forward to learn what I can at these workshops and bring this knowledge back and teach the people there,” said Naomi Billimon, a student at the Northern Marianas college in Saipan and an intern with the CNMI Division of Fish and Wildlife.

Guam NSF EPSCoR bolsters regional research capacity through fish ageing workshop

Fish Ageing Photo 2

On Monday, May 21, 2024, University of Guam graduate students, members of the Guam Green Growth Conservation Corps, as well as visiting scientists from Saipan, Palau, and Hawai’i convened at the UOG Marine Laboratory to learn how to determine the age of fish.

The fish ageing component was the first segment of the two-part, weeklong Fish Life History Workshop Series funded by Guam NSF EPSCoR to improve regional research capacity within Micronesia.

To determine the age of a fish, scientists extract a bonelike structure called an otolith from its ear and study its growth rings.

“Otoliths grow throughout time just like a tree. If you cut one in half, you can count the rings that are being laid down every single year,” said Brett Taylor, Ph.D., the workshop facilitator and an assistant professor of fisheries ecology at UOG. “In the hottest part of the year, these rings are growing the fastest, and in the coldest part of the year, they’re growing slower and creating a denser profile along the banded structures.”

According to Taylor, around 300 fish species are harvested in Guam. Knowing how to ascertain the age of a fish is vital to habitat conservation, fisheries management, and maintaining the perpetuation of local fishing practices and culture.

Many of the participants, such as Christina Muller-Karanassos, a researcher from the Palau International Coral Reef Center, had varying levels of experience regarding extracting, processing, and reading otoliths.

“I hope I can use this information in the future because we want to collect life history information for more fish species,” said Muller-Karanassos. “Nobody where I work really does any fisheries work, so I hope I’ll be able to pass on the knowledge to some people there.”

As part of the workshop, participants gained experience extracting otoliths from a variety of reef fish species at different life stages to hone their skills.

“The next part, which will be taught by our colleagues from Hawaii, will cover the histology of fish gonads. These workshops are putting together the two data pillars of life history information which are how old the fish are and what their maturity status is. When you put those two elements together, you can piece together the biology of the species,” said Taylor.

UOG alumna studies red algae in Saudi Arabia

MacKenzie Heagy

MacKenzie Heagy, a 2023 Master of Science in Biology alumna and former Guam NSF EPSCoR Graduate Research Assistant, gained valuable research experience as part of a six-month internship held at the King Abdullah University of Science and Technology (KAUST) in Saudi Arabia.

Each year, the Visiting Student Research Program at KAUST offers 130 students from more than a hundred countries the opportunity to develop their research skills, receive mentorship, and collaborate with subject-matter experts in world-class facilities.

Heagy heard about the program through colleagues who have met people from KAUST at different conferences and events.

During the program, Heagy studied red algae in the Red Sea in the Global Change Ecology Laboratory to understand the effects of global warming on key marine calcifying organisms.

“EPSCoR has helped me get connected to the community here in KAUST,” said Heagy. “It has given me a starting point for further research. I have developed a sense of initiative during my time on Guam and have grown exponentially as a scientist, student, and person. I am really proud of the person Guam and the UOG Marine Lab have pushed me to be.” 

Visiting scientists enrich Guam’s local community through research symposium

Bioblitz Symposium Photo 3

As part of the University of Guam’s first Bioblitz, an event focused on finding and identifying as many species as possible in a place over a set period, a team of international scientists engaged the island’s local community through a mini research symposium held on Friday, February 16, 2024.

Due to climate change, development, pollution, and other factors that contribute to habitat loss, the world is facing a biodiversity crisis. The first step in addressing this issue involves developing an understanding of what lives in the biosphere, the parts of the world where life exists, and how they function.

These scientists, who hail from the United States, Italy, and Japan, held a series of presentations concerning organisms such as mollusks, worms, shrimps, and other invertebrates to increase local research capacity and generate interest in these under-studied fields.

One of the presenters was Florida Museum Curator of Invertebrates Gustav Paulay, Ph.D., who formerly served as the director of the UOG Marine Laboratory.

“It can be disheartening to get into the water after a couple of decades and see what has happened to the reef,” said Paulay.” Some parts are great, some parts not so much. We are going through a biodiversity crisis and one thing that scientists can do about is at least get an idea of what the biosphere is like before it changes any further.”

During his time at UOG, Paulay spent years cataloging the diversity of invertebrates around Guam. The Bioblitz, supported by Guam NSF EPSCoR, was meant to expand the existing knowledge of Guam’s marine ecosystems to inform future management efforts and apply that knowledge to other kinds of studies.

“UOG sits in one of the most diverse areas and has this rich tradition of looking at organismal diversity. As a result, this institution is one of the centers of this work,” said Paulay. “We know what we know now fairly well because of a lot of effort done by a lot of people who have documented what they have described.”

UOG scientists find new marine biological records and species

Bioblitz Overview Photo 1

Following a three-week series of biological surveys, scientists from the University of Guam Marine Laboratory along with an international team of researchers estimate that they have collected hundreds of new marine biological records and species combined from around the coasts of Guam.

A Bioblitz is an event focused on finding and identifying as many species as possible in a place over a set period. This Bioblitz was supported by the university’s Guam National Science Foundation EPSCoR grant, which focuses on studying the biodiversity of marine life in Micronesia and how climate change affects the region.

From the southern coast of Guam almost up to Ritidian, the researchers surveyed around 50 sites through dives, intertidal walks, and snorkeling from February 2 – 22, 2024 and collected organisms such as crabs, shrimps, worms, and mollusks.

Over a thousand species were collected. According to UOG Biorepository Curator of Crustacea, Dr. Robert Lasley, an estimated 10 to 15 percent are new species or new records for Guam. A new biological record refers to an organism that has never been recorded within a geographic area.

“The findings from this Bioblitz are a testament to the University of Guam’s status as a premier research hub serving the Micronesian region,” said UOG President Anita

Borja Enriquez. “Through collaboration, dedication, and their commitment to discovery, these researchers have helped uncover the vast biodiversity living in the waters and sands of our island.”

Notable findings from the Bioblitz include:

  • A new record of a false spider crab found near the UOG Marine Laboratory
  • 71 new records of amphipods, a small shrimp-like crustacean
  • An estimated 46 new records of ribbon worms
  • Rare species of mollusks and gobies that live in symbiosis with a shrimp that inhabits underground burrows in the reef flats of Guam

Representatives of each species collected during the Bioblitz will be housed at the Guam NSF EPSCoR Guam Ecosystems Collaboratorium for Corals and Oceans Biorepository, a collection of marine fauna and flora that serves as an archive of the biodiversity found within the Micronesian region.

“After many long nights at the marine laboratory carefully processing specimens, these scientists have contributed immensely to our knowledge of this region’s ecosystems,” said Guam NSF EPSCoR Principal Investigator Dr. Terry Donaldson. “Through the Biorepository, we are able to share these findings with fellow researchers and work together to learn more about the world around us.”

Some specimens may be loaned to other scientists so that they can be examined further. The Biorepository team is partnering with Dr. Christopher Meyer from the National Museum of Natural History at the Smithsonian Institution to get all of the species DNA barcoded for identification.

Due to factors that impact marine ecosystems such as climate change, pollution, changing currents, and development, biological surveys serve as the first step towards preserving these ecosystems and the organisms that call them home.

“Right now, we’re experiencing a biodiversity crisis. We’re losing organisms and species very fast,” said Lasley. “How can you protect what you don’t know exists? It’s important to do this kind of work to capture this baseline data of what organisms are out there and where they live so we can try to build a foundation of knowledge that we can use to protect them.”

UOG graduate student heads to Okinawa to study marine invertebrates

Okinawa Internship Photo

A graduate student from the University of Guam is studying marine invertebrates as part of an internship with University of the Ryukus from April to August 2024.

Joseph Proietti, a Master of Science in Biology student and Guam NSF EPSCoR Graduate Research Assistant, is being mentored by James Reimer, a professor at the University of the Ryukus whose research focuses on the biodiversity of understudied marine invertebrates.

As part of the internship, Proietti is studying Palythoa, a type of cnidarian closely related to corals and anemones.

Some Palythoa, like hard corals, have symbiotic algae that live inside them that photosynthesize and feed their host in exchange for shelter and protection. These Palythoa are known to live in places with light availability such as open reefs while those without these symbionts live in caves or reef crevices.

“We’re going to take the Palythoa that have symbionts and the ones that don’t to do what’s called a reciprocal transplant experiment, which will involve switching them between their two habitats,” said Proietti. “Then we’ll monitor to see how this change in environment affects how they function depending on which of their genes are expressed.”

Besides networking with other professionals within his field, Proietti will learn skills that will help him advance as a scientist.

“During this internship, I’ll learn about RNA library preparation, a very specific type of lab work that involves preparing RNA to be sequenced which is complicated and challenging to get right,” said Proietti. “I’ll also learn how to analyze gene expression data.”

Proietti said he looks forward to exploring the reefs of Okinawa during this experience.

“I believe that traveling and experiencing as much of the world as you can is hugely beneficial to expanding your worldview and teaching you things that are hard to put into words,” Proietti. “These experiences are beneficial to not just your professional life, but who you are as a person.”

2023 GRA: Meet our graduate research assistants!

DSC01263

Since joining the Guam NSF EPSCoR Graduate Research Assistantship program, these graduate students will receive mentorship, training, and fieldwork experience as they pursue their master’s degree over the next three years.

 

ANELA DUENAS

“What made me want to get into marine science is when I got to experience a summer program as an elementary student and I was able to look at coral under a microscope at one of the beaches in Saipan,” said Anela Duenas. “I got to see the coral and how they move and how they’re alive and not rocks in the ocean. That experience inspired me and that made me keep going until now.”

While pursuing her undergraduate degree, Duenas was an NSF INCLUDES: SEAS Islands Alliance fellow as well as a Guam NSF EPSCoR student researcher.

Duenas joined the GRA program to encourage her peers to enjoy research. During her time in the Student Research Experience program, she received mentorship and guidance from a GRA named Justin Berg.

“Working with Justin was really fun and how he helped me as an undergrad encouraged me to help others and do what he did,” said Duenas.

Under the mentorship of Bastian Bentlage, Ph.D., an associate professor of bioinformatics and co-principal investigator of Guam NSF EPSCoR, Duenas is looking into assemblages of Symbiodiniaceae in two coral species – Acropora pulchra and Pavona decussata.

As a CHamoru scientist, Duenas hopes to inspire others within the region to pursue careers in STEM.

“I’ve felt personally connected to the land and the ocean ever since I was growing up, so it’s really cool for me to actually be working in this field and try to better our environment,” said Duenas. “I feel like I don’t see as many local scientists from here and

doing work here, so I hope I get to do some great work and hopefully inspire other locals in this area.”

 

DIANA NOTO

Diana Noto has always liked the ocean.

“I grew up on Long Island, so I lived in proximity to the water,” said Noto. “They weren’t particularly beautiful or clear beaches, but I always loved it. When I moved to Florida, I didn’t get to be closer to the water, but I got to go to the beach a lot more and get exposed to coral reefs. That’s how I got interested in the marine sciences.”

Noto heard about the program through Garret O’Donnell, a GRA who joined the program in 2022.

“Before he joined the program, Garret had also worked at the Florida Museum in the same lab as me,” said Noto. “When I was applying, I heard that Dr. Robert Lasley, who I worked with before, was taking on a student so I decided to just go for it.”

Robert Lasley, Ph.D., is a crustacean biologist and the associate curator of the Guam Ecosystems Collaboratorium for Corals and Oceans (GECCO) Biorepository, a natural history collection of specimens operated by Guam NSF EPSCoR.

“The program’s been really fun so far,” said Noto. “All the other students have been super great. I’ve learned a lot of cool things. Just being near so many cool sites to do fieldwork has been pretty amazing.”

 

NIKKO GALANTO

Nikko Galanto’s interest in marine biology was fostered in his junior year of high school.

“My teacher was really influential, and she taught us that Guam is unique and that whatever we do on the island has a huge impact on our surrounding oceans,” said Galanto.

Galanto heard about the GRA program in 2019 while he was pursuing his undergraduate degree. During that time, he became a student researcher as part of Guam NSF EPSCoR’s Student Research Experience and eventually went on to work as a DNA barcoding technician at the GECCO Biorepository.

“I thought that since graduate school was a part of my plans and that I’ve always been a part of EPSCoR, I decided to apply to the GRA program,” said Galanto. “I just hope to become a more well-rounded scientist. I’ve learned now that there’s so much I don’t know and that’s a good thing. “

Under the mentorship of Diego Vaz, Ph.D., a fish morphologist and the associate curator of the GECCO Biorepository, Galanto is exploring the systematics and morphology of blenny fish. Blenny fish are small, elongated fish that can be found on the ocean floor, in reefs, or in burrows.

As someone who grew up on Guam, Galanto said that his perspective as a local scientist gives him a really great insight into both the island’s environment and its community.

“I want to emphasize that we need a lot of representation in the field of STEM,” said Galanto. “I think it’s important to see POC in all walks of science and I think that’s

important because we all have different experiences – especially if we come from different islands and backgrounds. We all have something to contribute.”

UOG launches its first international marine biological survey

Bioblitz Photo 3

The University of Guam launched its first Bioblitz, an international collaboration to catalog the diversity of marine organisms found along the coasts of Guam from February 2 – 22, 2024.

In a ceremony held Thursday, February 1, at the UOG cliffside, eight visiting marine scientists were welcomed by UOG staff, faculty and some of the island’s elected officials.

“I look forward to what your research says and whatever recommendations and outcomes come forward so that we can continue our own food sustainability and preservation for our future and our children,” said Gov. Lou Leon Guerrero.

A Bioblitz, also known as a biological inventory, is an event that focuses on finding and identifying as many species as possible in a specific area within a short time. Due to climate change, many species around the world are at risk of extinction and Bioblitzes provide an opportunity to inform future conservation efforts of at-risk species.

“We have the most diverse coral reefs in the U.S. We have more species of corals and more species of fishes, and we have a lot of stuff that we don’t even know we’ve got yet. That’s up to the people who have come to participate in this Bioblitz to find out what those things are,” said Guam NSF EPSCoR Principal Investigator, Dr. Terry Donaldson.

The Bioblitz is supported by the university’s National Science Foundation EPSCoR grant, which aims to employ cutting-edge methods to determine solutions that address

the challenges imposed by climate change on coral reefs and associated ecosystems.

The visiting scientists include:

· Dr. Gustav Paulay, Florida Museum

· Dr. Justin Scioli, Smithsonian Marine Station in Florida

· Dr. Kristine White, Georgia College & State University

· Dr. Barbara Mikac, University of Bologna

· Dr. Svetlana Maslakova, University of Oregon

· Dr. Ryutaro Goto, Kyoto Museum

· Shawn Wiedrick, Los Angeles County Museum

· John Slapcinsky, Florida Museum

Along with researchers from the UOG Marine Laboratory, the scientists will collect specimens such as crustaceans, worms, and mollusks through dives, intertidal walks, and by snorkeling. For this event, local community members, scientists, and fishers were consulted to develop a list of locations to collect specimens.

“I would like to acknowledge and thank everyone who is interested in protecting and restoring Guam’s incredible marine ecosystems and those who just appreciate it and value it,” said UOG Biorepository Curator of Crustacea, Dr. Robert Lasley. “All forms of knowledge are important and play a role in our endeavor to protect and restore this biodiversity.”

Once the specimens collected during the Bioblitz have been processed, they will be placed in the Guam NSF EPSCoR Guam Ecosystems Collaboratorium for Corals and Oceans Biorepository, a natural history collection of marine fauna and flora that serves as an archive of the biodiversity found within the Micronesian region.

“This island has been inhabited for thousands of years and it was made habitable by the ocean around us,” said Dr. Paulay, curator of invertebrates at the Florida Museum and a former director of the UOG Marine Laboratory. “The CHamoru people relied on the marine resources to keep going in a place like this. For an island community, there’s nothing more important than the ocean, so understanding the ocean is absolutely fundamental to island culture and to maintain life on the island.”

UOG grad student represents Guam in international fish conference

Fish Conference 2023 Photo 2
Fish Conference 2023 Photo 2

Karina Mejia, a Guam NSF EPSCoR Graduate Research Assistant in the University of Guam’s Master of Science in Biology program, presented her research at the 2023 Indo-Pacific Fish Conference which was held at the University of Auckland in New Zealand.  

The Indo-Pacific Fish Conference, which happens every four years, is one of the world’s premier ichthyological conferences and is highly anticipated by marine, estuarine, and freshwater specialists. The event is organized by the Australian Society for Fish Biology, which aims to promote research, education, and management of fish and fisheries across the Indo-Pacific.  

Mejia’s presentation was entitled, “Population Genetics of the Catadromous Eel, Anguilla marmorata, in Guam and the Indo Pacific.”  

Giant mottled eels (Anguilla marmorata) are the most common eels in the Indo-Pacific and Guam. They are an important food fish and an apex predator in many tropical islands and continental streams of the Indo-Pacific, according to the study’s abstract. Determining the eel’s geological and oceanographic location and temporal spawning patterns will provide information key to effective conservation.  

 During the event, Mejia found it exciting to meet people within her field. She connected with a doctoral student named Yusuke Amai who invited her to explore doctoral programs at the University of Tokyo, which she was able to visit in December. While she was in Japan, Mejia met with Nina Yasuda, Ph.D., a professor at the University of Tokyo who has been helping her with the genetic portion of her research.  

“At first, I was intimidated since I was surrounded by experts, but I learned to not be scared and shy to interact with fellow scientists,” said Mejia. “I am glad I learned to overcome my nervousness since I was able to meet a variety of wonderful people and interact with potential mentors for a Ph.D.” 

Three represent UOG at international coastal sciences conference

Outlook 42qhsotc
A team from the University of Guam attends the 2023 Coastal & Estuarine Research Federation Conference (CERF) from November 12 - 16, 2023, in Portland, Oregon.  (From top left) Sarai Vega, Dr. Cheryl Sangueza, Anna Aguirre, Anthony Ritter, Tonya McDaniel, Lynn Galang, and Anela Duenas
A team from the University of Guam attends the 2023 Coastal & Estuarine Research Federation Conference (CERF) from November 12 – 16, 2023, in Portland, Oregon. (From top left) Sarai Vega, Dr. Cheryl Sangueza, Anna Aguirre, Anthony Ritter, Tonya McDaniel, Lynn Galang, and Anela Duenas
During the event, UOG students had the opportunity to interact with peers, network with scientists within their fields, and attend workshops and panel discussions.       This year’s delegation included Guam NSF EPSCoR Graduate Research Assistants Anela Duenas and Sarai Vega as well as Lynn Galang, a 2022 undergraduate student researcher.
During the event, UOG students had the opportunity to interact with peers, network with scientists within their fields, and attend workshops and panel discussions. This year’s delegation included Guam NSF EPSCoR Graduate Research Assistants Anela Duenas and Sarai Vega as well as Lynn Galang, a 2022 undergraduate student researcher.

Three student researchers from the University of Guam attended the 2023 Coastal & Estuarine Research Federation Conference (CERF), which was held from November 12 – 16, 2023, in Portland, Oregon.  

The conference is a biennial international meeting of coastal and estuarine scientists and managers that aims to advance the understanding and stewardship of these ecosystems worldwide.  

This year’s conference theme was “Resilience and Recovery,” which was chosen to reflect not only the scientific context of those terms but also the resilience of CERF’s community members through the COVID-19 pandemic.  

During the event, students had the opportunity to interact with peers, network with scientists within their fields, and attend workshops and panel discussions.  

This year’s delegation included Guam NSF EPSCoR Graduate Research Assistants Anela Duenas and Sarai Vega as well as Lynn Galang, a 2022 undergraduate student researcher.  

During the conference, Duenas presented her research poster entitled, “Ecophysiology of reef flat coral species replacement in the Mariana Islands.” Her study focused on analyzing endosymbiont communities within stony corals such as Acropora pulchra and Pavona decussata to gather insight about their role in coral resilience.  

“My CERF 2023 experience was amazing! I felt so inspired by all the research and people I met. I especially loved the CERF Conference because it was not too big of a conference where you could feel lost and out of place. Instead, the conference felt warm and welcoming like catching up with old friends,” said Duenas.  

Galang’s presentation was titled “Taxonomic and Genetic Diversity of the Corallivorous Snail, Drupella spp., on Guam.” Her study focused on determining what species of coral-eating snails are present on Guam to better inform coral management practices.  

For her poster presentation entitled, “A seasonal study of meiofauna distribution in Pago Bay, Guam,” Vega explored the world of meiofauna — invertebrates that live in marine and freshwater environments between sand and mud on the seafloor and riverbeds. Copepods, flatworms, and nematodes fall under meiofauna.  Vega’s study focused on how environmental changes affect meiobenthic communities

In a session called “Stories from the Braided River: Nonlinear, Enriching Workforce Pathways and Narratives in Coastal Science and Management,” Vega also delivered a presentation about her journey to graduate school in a session entitled, “How many turns does it take? My path to graduate school and beyond.”  

Vega’s oral presentation detailed the challenges and successes she faced navigating higher education as an international student, changing her major and choice of career, as well as working while taking classes.  

“I learned that when I have doubts in my abilities by taking a hold in the confidence other people have in me, I am able to make the first step,” said Vega. “It’s not “fake it till you make it” – it’s a lot better than that. It’s knowing that you have the support of your ancestors, generations behind you who have worked hard for us to get to where we are. It’s knowing that no matter what, my value as someone in science goes beyond what I can or cannot do. It’s understanding that in a safe environment, learning is bound to take place. “ 

Skip to content