Collector donates thousands of shells to UOG Biorepository

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EPSCoR 20250627 RobLasley ShellsMiniBlitz 8
Robert Lasley, Ph.D., assistant professor and curator of crustacea at the UOG Biorepository, opens some shell samples donated by collector Warren Carah.

A retired shell enthusiast recently donated thousands of shells to the Guam NSF EPSCoR Biorepository, many of which were collected on Guam during the 1960s, to be added to its historical collection.

Warren B. Carah, author and engineer, spent his teenage years in Guam from 1960 to 1964, when his family relocated due to his father’s service as an officer in the U.S. military. Carah attended Tumon High School, now known as John F. Kennedy High School, and spent his after-school hours searching for shells in Tumon Bay with his friends.

“We went to our lockers, got our spear guns out, and took the old Japanese elevator that went  from the cliff down to the beach and we spent the rest of the day shelling, we went to just about every beach on the island,” Carah said.

Aside from Tumon Bay, Carah and his friends also frequented Apra Harbor, Malesso’, Cocos Island, and Tarague Beach in Andersen Air Force Base. 

“I used to spend many, many hours out there at night. The bottom would literally be crawling with the very large cone shells, olive shells, and cowrie shells and during the day, it would look barren but at night, everything had come out,” Carah stated. 

The donated shell collection includes around 4,000 shells from Guam, the Philippines, Australia, North America, and Africa. 

Carah is aware of the difference in shell conservation now compared to how it was in the 1960s. “I think nowadays, most anybody that collects shells on Guam is quite aware of the fact that they have to let that resource go after they had found it, photographed it perhaps.,” Carah said, adding, “That didn’t exist in the early 1960s, but we still practiced conservation. There was no point in us collecting dozens and dozens of the same shell. We would collect one or two and our efforts would then go to find a new species.” 

The Guam NSF EPSCoR Biorepository works to consolidate and expand Guam’s natural history collections and provides state-of-the-art digitization, imaging, and mapping of local and regional marine fauna and flora.

Regarding the importance of this donated shell collection, Robert Lasley, Ph.D., assistant professor and curator of crustacea at the UOG Biorepository, describes it as “valuable for establishing a historical baseline, as well as for studying different species to better understand Guam’s biodiversity. It also allows researchers to compare what may have existed in certain localities in Guam back in the ‘60s to what is being found now.”

Carah had been sitting on his shell collection for almost 60 years before ultimately deciding to donate it to a place that can benefit from its possession.

“I sent a letter to the dean of the biological sciences group there at the University of Guam and she evidently then turned that over to Dr. Lasley, and then he contacted me via email and we’ve been corresponding ever since,” Carah said. 

Lasley stated that Carah’s shell collection includes the exact day it was collected and the precise location where it was gathered. “So he not only did these collections, but he kept a lot of good data and took really good care of them. In fact, you can see how well he packaged this stuff and sent it to us in really good condition,” he said.

The Biorepository houses thousands of coral specimens, crustaceans, fishes, algae, and other organisms to serve as an archive of the biodiversity found within the Micronesian region. Lasley explained that modern tools now allow for the collection and global dissemination of data from these specimens. He added that, eventually, all of them will be photographed, and their locality data will be gathered and entered into the curatorial database

“We’re going to get all the locality data from all these, and we’ll put them into our curatorial database, but then serve them online globally so any researcher anywhere in the world can access this information,” Lasley said.

Guam EPSCoR’s Lobban receives award from Japanese diatom society

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In 2024, Guam NSF EPSCoR’s Dr. Christian Lobban received an award from the Japanes Society of Diatomology for his paper “Disymmetria reticulata, gen. nov., sp. nov. (Mediophyceae: Thalassiosirales), a new genus in Lauderiaceae emend., and transfer of Lauderia excentrica”. Lobban is the first non-Japanese member of the organization to receive an award. 

 

In “Disymmetria reticulata, gen. nov., sp. nov. (Mediophyceae: Thalassiosirales), a new genus in Lauderiaceae emend., and transfer of Lauderia excentrica”, Lobban proposes the creation of a new genus Disymmetria  to accommodate two species of diatom that are morphologically close to each other. 

 

“One of the things that they liked about the paper was that it dealt with some of the difficulties, the complexities of naming genera and families,” said Dr. Lobban. He notes that taxonomic classification of newly discovered species can be difficult, particularly in the determination of taxonomic levels such as family or genus. 

 

This paper is an update to the discovery of the diatom Lauderia excentrica in 2015, in which Lobban originally classified the species under the genus Lauderia

 

“When you find a new species, then you’ve got this choice. It doesn’t look like anything else really. Do I put it in an existing genus? And there was an existing genus but it didn’t really fit,” said Lobban of the discovery. Lobban had decided to classify Lauderia excentrica under its original genus rather than create a single species genus. However, in 2023 he discovered another diatom that shared enough characteristics to justify the creation of a new genus.

 

In his 2023 paper, Lobban suggested that Lauderia excentrica should transfer to the new genus along with the new species Disymmetria reticulata. The research was published in DIATOM, the official journal of the Japanese Society of Diatomology. Lobban also traveled to Japan to receive his award along with a special mug with “Best Paper Award 2024” written across it.

 

Diatoms are single-celled algae that can be found in almost every aquatic environment and produce an estimated one-fifth of the oxygen in the air that we breathe. Because the organisms are microscopic, a scanning electron microscope is required to view them. 

 

Through his research and mentorship, Dr. Lobban has made significant strides in the discovery of dozens of species of diatoms within the Pacific. In addition to his research, Dr. Lobban is also a mentor for Guam EPSCoR’s Student Research Experience program. Currently, he is working with Undergraduate Student Researcher Maya Flores on her project “Underexplored Sand Biofilm Habitats of Mastogloia in Guam”.

In 2024, Guam NSF EPSCoR senior researcher Christopher Lobban, Ph.D., received an award from the Japanese Society of Diatomology for his paper “Disymmetria reticulata, gen. nov., sp. nov. (Mediophyceae: Thalassiosirales), a new genus in Lauderiaceae emend., and transfer of Lauderia excentrica.” Lobban, a UOG professor emeritus of biology,  is the first non-Japanese member of the organization to receive an award. 

In “Disymmetria reticulata, gen. nov., sp. nov. (Mediophyceae: Thalassiosirales), a new genus in Lauderiaceae emend., and transfer of Lauderia excentrica,” Lobban proposes the creation of a new genus Disymmetria  to accommodate two species of diatom that are morphologically close to each other. 

“One of the things that they liked about the paper was that it dealt with some of the difficulties, the complexities of naming genera and families,” said Lobban. He notes that taxonomic classification of newly discovered species can be difficult, particularly in the determination of taxonomic levels such as family or genus. 

This paper is an update to the discovery of the diatom Lauderia excentrica in 2015, in which Lobban originally classified the species under the genus Lauderia

“When you find a new species, then you’ve got this choice. It doesn’t look like anything else really. Do I put it in an existing genus? And there was an existing genus but it didn’t really fit,” said Lobban of the discovery.

Lobban had decided to classify Lauderia excentrica under its original genus rather than create a single species genus. However, in 2023 he discovered another diatom that shared enough characteristics to justify the creation of a new genus.

In his 2023 paper, Lobban suggested that Lauderia excentrica should transfer to the new genus along with the new species Disymmetria reticulata. The research was published in DIATOM, the official journal of the Japanese Society of Diatomology. Lobban also traveled to Japan to receive his award along with a special mug with “Best Paper Award 2024” written across it.

Diatoms are single-celled algae that can be found in almost every aquatic environment and produce an estimated one-fifth of the oxygen in the air that we breathe. Because the organisms are microscopic, a scanning electron microscope is required to view them.

Through his research and mentorship, Lobban has made significant strides in the discovery of dozens of species of diatoms within the Pacific. In addition to his research, Lobban is also a mentor for Guam NSF EPSCoR’s Student Research Experience (SRE) program. Currently, he is working with undergraduate student researcher Maya Flores on her project “Underexplored Sand Biofilm Habitats of Mastogloia in Guam.”

UOG welcomes researchers for coral restoration collaboration

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In April 2025, the University of Guam welcomed visiting researchers as part of an ongoing collaboration on the development of a new research instrument that can be used to assess the bleaching risk of different types of coral. 

The instrument, called a chlorophyll fluorometer, is a non-destructive and non-invasive tool that uses different colors of light to measure the photochemical response of symbiotic algae within reef building coral. 

During the visit, researchers Mark Warner, Ph.D., professor of marine science and policy at the University of Delaware, and Sophie Wong, a graduate researcher at the University of Alabama, were able to use the fluorometer to test the thermal stress response of different species within Guam’s waters. The researchers aimed to use the instrument on coral that have lived through previous bleaching events to observe which species may be more tolerant to conditions that lead to bleaching. 

“We can actually measure really fine scale differences in the symbiotic algae inside of the reef building corals,” said Warner. “A lot of the times heat stress causes the algae to stress out first.” 

According to Warner, use of the fluorometer can make it possible to observe thermal stress before symptoms of bleaching appear on the coral visually, allowing researchers to get ahead of coral reef management. Additionally, the instrument will help to observe characteristics of coral physiology which may indicate species that are more resilient to severe environmental conditions.

“This is meant to make trait-based restoration more accessible,” said Wong who works alongside the project’s lead principal investigator Kenneth Hoadley, Ph.D., who has been developing this instrument over several years. “So basically, identifying which corals are going to be more thermally tolerant based on the traits that they have. This basically measures those traits and is able to tell you, oh, this one has a trait that would be more conducible to surviving a bleaching event.”

“The hope is to use this to then identify those corals that are more resilient to increasing sea surface temperatures, to heat stress,” said Guam NSF EPSCoR’s Bastian Bentlage, Ph.D., who is one of the project’s co-principal investigators.

The use of the fluorometer is a ground-breaking method of gathering this type of data on the coral, which would otherwise require physically extracting tissue and DNA out of the algae within the coral — a much more intensive process.

As part of the research, this bio-optical instrument has also been tested in Fiji. Warner notes that a collaboration in Guam has many benefits, including being able to sample coral species that are unique to Guam, as well as species they were not able to access in Fiji. 

“The University of Guam Marine Lab has been just really fantastic. They’ve facilitated a lot of things,” commented Warner, noting that the coordination of the University Marine Lab allowed their field work to run smoothly. “And so, I think it’s going to be a really fun collaboration moving forward.”

As the project progresses, the researchers will continue to test the instrument on a variety of coral species as well as run coral bleaching simulation tests. New prototypes of the instrument will also continue to be developed as a means to ensure that the technology is accessible to researchers world-wide who are dedicated to the management and restoration of these invaluable coral reefs.

This research project, funded by the Coral Research and Development Accelerator Platform, is being conducted in collaboration with coral researchers Hoadley of the University of Alabama; Warner of the University of Delaware; Bentlage and Laurie Raymundo, Ph.D., of the University of Guam; and Victor Bonito, Ph.D., of the nonprofit organization Reef Explore Fiji.

Kallen presents research findings on coral-eating sea snails

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University of Guam NSF EPSCoR Graduate Research Assistant Lauren Kallen defended her Master’s thesis—a research project that represents the first study of its kind on Guam, focusing on understanding the population dynamics of Drupella fragum, a sea snail that feeds on coral tissue and can be found on shallow reef flats around the island.

Kallen defended her  thesis, “Genetic Characterization of the Corallivore Drupella fragum Outbreaks on Guam,” on April 29, 2025. 

The goals of this study were to observe the behavior and distribution of the Drupella species in order to understand population outbreaks and explore mitigation strategies. For her study, Kallen observed the effects of temperature changes on the species’ reproductive outputs as well as genetic diversity within outbreak populations. 

A Drupella fragum outbreak is quantified by finding 2 or more individuals per metered square. During her research, Kallen observed high numbers of the species of sea snail during outbreaks at Agat cemetery. 

“It would actually take a while to find a single snail so finding more than one was a huge indicator of an outbreak,” said Kallen during her presentation. “[The highest number observed] was hundreds within a small area, clumped together, which was kind of crazy to see.”  

In her research, Kallen found that populations between two observed sites in Tanguisson and Agat were genetically interconnected, yet outbreaks were only observed in the Agat cemetery location. Kallen notes that Tanguisson is a healthier site overall than Agat cemetery, particularly in terms of coral condition and sedimentation. While outbreak conditions still need to be studied further, Kallen notes that it is possible that the overall health of the site may explain the absence of outbreaks at Tanguisson beach. 

Along with coral tissue, the Drupella snail also feeds on coral mucus which corals secrete as a stress response to protect themselves from severe environmental conditions. Kallen notes that it is possible that coral mucus secretion may serve as a cue that attracts the Drupella to certain areas.  

There have been several studies on the species worldwide but because there has been no other research on Drupella outbreaks on Guam, Kallen referred to studies on Crown-of-thorns starfish outbreaks to help shape her research and methodology.  

“Hopefully, my work will inspire future studies that expand on these findings by testing more environmental variables and conducting further genetic analyses on these coral-eating snails in Guam,” said Kallen. 

She hopes that her study will encourage researchers to do further analysis on environmental and genetic factors of species outbreaks. While there is still more to learn about Drupella fragum outbreaks, Kallen hopes that it will provide a stepstone for more research on the species to help protect Guam’s vulnerable reefs.  

Kallen’s research is supported by Guam NSF EPSCoR as part of its Graduate Research Assistantship program, funded by the National Science Foundation, with aims to situate Guam as a premiere research and STEM education hub.  

UOG EPSCoR GECCO Biorepository partners with Smithsonian Institute for DNA sequencing

Guam NSF EPSCoR Biorepository Associate Abigail Huber collecting tissue samples from specimens to send to the Smithsonian Institution for DNA sequencing.
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A sample DNA sequence for a specimen at the Guam NSF EPSCoR Biorepository. The color of the line corresponds to the amino acid (coded A, T, G or C) that has been identified for the specimen.

The University of Guam NSF EPSCoR GECCO Biorepository has partnered with the Department of Invertebrate Zoology at the National Museum of Natural History, Smithsonian Institution, Washington, D.C., for the DNA sequencing of marine organisms.  

The Smithsonian Institution is currently working on a project to DNA barcode marine invertebrate species in US marine systems regions and has partnered with the UOG EPSCoR Biorepository to barcode Guam’s marine invertebrate species. Because Guam has more species of organisms than any other marine region in the United States, this partnership is beneficial for both institutions.  

According to Biorepository Curator of Crustacea Robert Lasley, Ph.D., species identification is an intensive process that often requires hours of examining specimens under a microscope and delving into hundreds of years literature on any given organism.

He said it can be particularly difficult to identify the species that are found within any marine system, especially for understudied or obscure organisms such as marine worms, brittle stars and small crustaceans. DNA barcoding is an easier and more effective method way of identifying organisms, by matching a newly generated sequence of one gene to sequences of the same gene that were previously identified and in a barcode database.

“With DNA barcoding, it is possible to sequence one gene of a specimen, compare it with a library of sequences of known species, and arrive at an identification without spending (potentially) years becoming an expert in the particular type of worm you are trying to identify,” said Lasley. 

“Sometimes we aren’t able to identify specimens, just by looking at it – either because the differences (between species) are too small for us to see or because we haven’t found the distinguishing characteristic yet,” said Biorepository Research Associate Abigail Huber, who has been collecting tissue samples from the field and preparing them to be sent to the Smithsonian Institution.

The tissue samples undergo a laboratory process that isolates the DNA and a sequencing machine determines the order of the amino acid bases that make up the DNA molecule. This string of amino acids (coded A, T, G or C) of one gene is the barcode.  

Each DNA sequence is then reviewed by Huber, who verifies that each specimen was sequenced properly. Because errors can arise in the sequencing process, quality control is one of Huber and other members of the Biorepository’s responsibilities. 

Guam NSF EPSCoR Biorepository Associate Abigail Huber collecting tissue samples from specimens to send to the Smithsonian Institution for DNA sequencing.
Guam NSF EPSCoR Biorepository Associate Abigail Huber collecting tissue samples from specimens to send to the Smithsonian Institution for DNA sequencing.

“In this case, we look at other specimens that we’ve sequenced that are the same species and compare this section of DNA with theirs to determine what the correct amino acid is,” said Huber. “Species of organisms will have the same (or similar) DNA sequence, which works great for comparing.”

As of April 2025, around 3,100 specimens have been sent for sequencing at the Smithsonian Institution and about 1,500 sequences have been received by the Biorepository. These barcodes are currently being quality controlled and will be eventually uploaded to the Biorepository online database so that researchers from around the world will be able to access this data remotely.

The DNA barcodes generated in this partnership, and the associated new specimens in the Biorepository, will result in the discovery of new species and will aid in research in a variety of fields such as ecology, conservation, fisheries and evolution.

Behind the Guam EPSCoR library of life

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Off the shores of Guam is one of the most complex marine systems in the United States, home to thousands of organisms – many of which are still being documented and identified to this day. The Guam NSF EPSCoR GECCO Biorepository is part of an on-going effort to understand those organisms as well as their habitats.

What can also be described as a “Library of Life,” the Biorepository is a physical and cyber warehouse that houses specimens and data on organisms from waters throughout Micronesia and beyond. The biological specimens include species from historical collections, such as the 29,0000+ specimen coral collection of the late UOG Emeritus Professor Richard Randall, and organisms from more recent EPSCoR-funded research projects, such as last year’s Bioblitz.

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Guam NSF EPSCoR Biorepository Research Associate David Burdick examines specimens at the Biorepository Lab.

“Ultimately, what we’re trying to do is just come up with specimen data that’s world class that a lot of museums don’t have for the organisms in their possession,” said Guam NSF EPSCoR Biorepository Research Associate Dave Burdick. Burdick notes that one of the advantages to the Biorepository on Guam is that the team has easier access to reefs for field collection, compared to other researchers in the United States.

“We have that advantage to be able to dig deeper and better capture that habitat data. That, I think, helps set us apart,” said Burdick. “And even though we’re kind of new and relatively small, I think that could be really powerful for researchers.”

Burdick, along with the rest of the Guam NSF EPSCoR Biorepository team, has been working tirelessly to catalog thousands of specimens for both the physical and online components of the Biorepository. Just the Randall coral collection alone has taken several years of work since that data needed to be transcribed from Randall’s original writing dating back to the 1960s.

The team has been working to upload all of the data from the collections onto an online portal which they hope to have fully updated and ready for public use soon. The physical specimen is given a catalog number, labeled and housed in the EPSCoR Annex in the UOG Dean’s Circle.

“When every specimen has been correctly labeled,” said Abigail Huber, a research associate who joined the team in 2024, “I transport all the jars to the annex and arrange them from family down to genus and sometimes species. We had about 2,000 [recently collected] specimens to go through so this took a long, long, long, time.” These specimens can be loaned out to researchers who may be interested in a specific species to study.

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Guam NSF EPSCoR Biorepository Research Associate Abigail Huber collecting tissue samples from specimens.

However, there are still many specimens that have yet to be properly identified, including many from the 2024 Bioblitz. Identifying these species is an intensive process which requires the researchers to look through available literature in an attempt to properly name each specimen.  Fortunately, the Biorepository has begun a partnership with the Smithsonian Museum in Washington D.C. to identify the specimens through DNA sequencing. Huber, who has been working on both sorting the specimens and sending tissue samples, notes that DNA sequencing will help researchers identify organisms more efficiently.

Being able to identify these organisms and their homes is important for conservation efforts so that communities can better understand how to protect them, as well as help preserve the way of life for people in the region whose cultures depend on the ocean.

Duenas presents thesis on coral-friendly microalgae

Guam NSF EPSCoR Graduate Research Assistant Anela Duenas presents her thesis proposal "Ecophysiology of Coral Species Replacement on Reef Flats of Guam."

In preparation for her master’s thesis defense this summer, Anela Duenas, a Guam NSF EPSCoR graduate assistant, presented her proposal, “Ecophysiology of Coral Species Replacement on Reef Flats of Guam,” that examines the relationship of the microalgae Symbiodiniaceae with reef-building corals Acropora pulchra and Pavona decussata.  

Symbiodiniaceae is a family of dinoflagellate algae notable for symbiotic associations with reef-building corals. Through her research, Duenas confirmed two genera of symbionts dominating A. pulchra – Cladocopioum and Durusdinium – and one dominating P. decussata – Cladocopium

In her presentation held in January at the UOG Marine Lab, Duenas noted the significance of the symbiotic relationship between coral and Symbiodiniaceae for the health and success of reef-building coral such as Acropora pulchra and Pavona decussata.

Duenas has begun working on her experiments under the mentorship of EPSCoR’s Bastian Bentlage, Ph.D., and will continue them over the next few months. She expects to start analyzing her results in March and to present her findings and defend her thesis in the summer.

As part of the process, Duenas will conduct thermal stress experiments on the two coral species that harbor these symbionts. The experiments are designed to simulate coral bleaching conditions, allowing her to observe how the corals and their accompanying symbiont variants react to and recover from thermal stress.

Through her experiments, she aims to document coral species replacement on Guam’s reef flats, assess the diversity of Symbiodiniaceae assemblages present, and understand how the observed corals harboring the symbionts respond to environmental changes.

“Presenting my proposal was a big step in the process. I am relieved it went well, and the audience raised important concerns and questions for me to consider moving forward,” said Duenas, expressing her appreciation for the discussion that followed her presentation. She and her committee carefully considered the feedback and adjusted her methods in ways she believes will improve her experiment.

“I feel more confident after meeting with my committee and addressing these concerns,” she remarked. “I am excited to move forward with my thesis project and conduct my experiment.”

Hailing from the island of Saipan, Duenas first became interested in marine science by observing coral under a microscope on the beaches where she grew up. She went on to earn a degree in natural resources management from Northern Marianas College before obtaining her bachelor’s degree in biology from the University of Guam.

Guam NSF EPSCoR Graduate Research Assistant Anela Duenas presents her thesis proposal "Ecophysiology of Coral Species Replacement on Reef Flats of Guam."
Guam NSF EPSCoR Graduate Research Assistant Anela Duenas presents her thesis proposal "Ecophysiology of Coral Species Replacement on Reef Flats of Guam."

In preparation for her master’s thesis defense this summer, Anela Duenas, a Guam NSF EPSCoR graduate assistant, presented her proposal, “Ecophysiology of Coral Species Replacement on Reef Flats of Guam,” that examines the relationship of the microalgae Symbiodiniaceae with reef-building corals Acropora pulchra and Pavona decussata.  

Symbiodiniaceae is a family of dinoflagellate algae notable for symbiotic associations with reef-building corals. Through her research, Duenas confirmed two genera of symbionts dominating A. pulchra – Cladocopioum and Durusdinium – and one dominating P. decussata – Cladocopium

In her presentation held in January at the UOG Marine Lab, Duenas noted the significance of the symbiotic relationship between coral and Symbiodiniaceae for the health and success of reef-building coral such as Acropora pulchra and Pavona decussata.

Duenas has begun working on her experiments under the mentorship of EPSCoR’s Bastian Bentlage, Ph.D., and will continue them over the next few months. She expects to start analyzing her results in March and to present her findings and defend her thesis in the summer.

As part of the process, Duenas will conduct thermal stress experiments on the two coral species that harbor these symbionts. The experiments are designed to simulate coral bleaching conditions, allowing her to observe how the corals and their accompanying symbiont variants react to and recover from thermal stress.

Through her experiments, she aims to document coral species replacement on Guam’s reef flats, assess the diversity of Symbiodiniaceae assemblages present, and understand how the observed corals harboring the symbionts respond to environmental changes.

“Presenting my proposal was a big step in the process. I am relieved it went well, and the audience raised important concerns and questions for me to consider moving forward,” said Duenas, expressing her appreciation for the discussion that followed her presentation. She and her committee carefully considered the feedback and adjusted her methods in ways she believes will improve her experiment.

“I feel more confident after meeting with my committee and addressing these concerns,” she remarked. “I am excited to move forward with my thesis project and conduct my experiment.”

Hailing from the island of Saipan, Duenas first became interested in marine science by observing coral under a microscope on the beaches where she grew up. She went on to earn a degree in natural resources management from Northern Marianas College before obtaining her bachelor’s degree in biology from the University of Guam.

UOG workshop reveals findings on tackling crown-of-thorns outbreaks

Dr. Ciemon Caballes, a senior research associate at the University of Guam's NSF EPSCoR and assistant professor at the UOG Marine Lab, presented findings from a study during the "Guam Crown-of-Thorns Starfish Outbreak Response Strategy" workshop in December at the UOG Marine Lab.
Dr. Ciemon Caballes, a senior research associate at the University of Guam's NSF EPSCoR and assistant professor at the UOG Marine Lab, presented findings from a study during the "Guam Crown-of-Thorns Starfish Outbreak Response Strategy" workshop in December at the UOG Marine Lab.
Dr. Ciemon Caballes, a senior research associate at the University of Guam's NSF EPSCoR and assistant professor at the UOG Marine Lab, presented findings from a study during the "Guam Crown-of-Thorns Starfish Outbreak Response Strategy" workshop in December at the UOG Marine Lab.

Insights into reproductive trends, predation patterns, and climatic triggers observed before a crown-of-thorns (COTS) outbreak are crucial for developing effective mitigation strategies to protect coral reefs, according to Ciemon Caballes, Ph.D., a senior research associate at the University of Guam’s NSF EPSCoR and assistant professor at the UOG Marine Lab.

Caballes shared his insights during the “Guam Crown-of-Thorns Starfish Outbreak Response Strategy” workshop in December at the UOG Marine Lab.

COTS are marine invertebrates known for their predation on corals. They can cause devastating impacts on coral reefs by depleting coral populations. They are found in reefs across the Indo-Pacific region.

Caballes’ research, supported by a grant from the National Fish and Wildlife Foundation, in collaboration with UOG’s NSF EPSCoR and Sea Grant, and other local and federal resource management agencies, focuses on understanding and mitigating these outbreaks.

“When we try to catch a serial predator, like the crown-of-thorns starfish, we need to build a profile,” Caballes said noting that understanding COTS and the trends leading to an outbreak is crucial for an effective mitigation strategy.

Caballes outlined several hypotheses regarding the formation of COTS outbreaks. The first suggests that outbreaks are a natural biological predisposition, given the species’ high fertilization rates.

Participants from the University of Guam and other groups listen to Dr. Ciemon Caballes, a senior research associate at the Guam NSF EPSCoR and assistant professor at the UOG Marine Lab, at the "Guam Crown-of-Thorns Starfish Outbreak Response Strategy" workshop in December at the UOG Marine Lab.
Participants from the University of Guam and other groups listen to Dr. Ciemon Caballes, a senior research associate at the Guam NSF EPSCoR and assistant professor at the UOG Marine Lab, at the "Guam Crown-of-Thorns Starfish Outbreak Response Strategy" workshop in December at the UOG Marine Lab.

According to Caballes, even a single mating pair can initiate a population surge. He said a female starfish could potentially produce over 100 million eggs in one spawning season while a male can produce around 50 billion sperm.

Surprisingly, he pointed out that, despite a male-skewed sex ratio, crown-of-thorns starfish maintain high fertilization rates, achieving up to 30% success even when separated by significant distances.

The second hypothesis links outbreaks to the overfishing of natural predators, leading to the release of COTS from predatory pressure and triggering a trophic cascade.

He also discussed the nutrient hypothesis, which posits that elevated nutrient levels from river runoff result in increased phytoplankton densities.  Since phytoplankton provide a source of food for COTS larvae, the spike improves their survival and development rates. However, he also said recent findings indicate that excessive phytoplankton might lead to overfeeding, affecting larvae survival negatively.

Caballes also recalled several significant outbreaks, particularly one in 2009, and noted the possible influence of temperature and strong El Niño-Southern Oscillation (ENSO) events on COTS populations. He illustrated the correlation between high precipitation and river discharge events, such as those in 2003 and 2004, and subsequent COTS outbreaks in 2006 and 2007.

Caballes noted, “With back-to-back coral bleaching events compounded by COTS outbreaks, corals are struggling to recover.” He emphasized the need for effective management strategies to address these dual threats.

“This workshop is the culmination of our work since 2022. The project began in March of that year to assess the crown-of-thorns starfish populations around Guam and explore the feasibility of coral rehabilitation in these reef areas.” Caballes stated.

Caballes described their research efforts, which included testing various plots to determine if culling crown-of-thorns starfish would enhance the survival of transplanted corals. 

Input from workshop participants have been integrated into the Guam COTS Outbreak Strategic Management Plan, which will be available to the public and distributed to all local and federal partners.

Fran Castro, associate director for operations and development at the UOG Center for Island Sustainability and Sea Grant, highlighted the significance of the project in addressing COTS. She described multi-agency efforts that involved training local agencies and coordinating monitoring efforts.

UOG’s Donaldson co-authors paper on fish that communicate with sound

Sargocentron spiniferum or Sabre Squirrelfish, the largest member of the family Holocentridae (Squirrelfishes and Soldierfishes), are able to communicate with sound as detailed in a scientific paper recently published and co-authored by Guam NSF EPSCoR’s Terry Donaldson, Ph.D., at the University of Guam. Photo credit: Dave Burdick/Guam Reef Life
Sargocentron spiniferum or Sabre Squirrelfish, the largest member of the family Holocentridae (Squirrelfishes and Soldierfishes), are able to communicate with sound as detailed in a scientific paper recently published and co-authored by Guam NSF EPSCoR’s Terry Donaldson, Ph.D., at the University of Guam. Photo credit: Dave Burdick/Guam Reef Life
Sargocentron spiniferum or Sabre Squirrelfish, the largest member of the family Holocentridae (Squirrelfishes and Soldierfishes), are able to communicate with sound as detailed in a scientific paper recently published and co-authored by Guam NSF EPSCoR’s Terry Donaldson, Ph.D., at the University of Guam. Photo credit: Dave Burdick/Guam Reef Life

University of Guam Professor of Ichthyology Terry Donaldson recently co-authored a paper on bioacoustic communication within the family Holocentridae, a group of marine fishes commonly known as squirrelfishes and soldierfishes. These fishes are known to communicate by sound production. The paper, “Sounds as taxonomic indicators in holocentrid fishes,” was a collaboration with Marine Banse, Eric Parmentier and Estrelle Bertimes (University of Liege, Belgium), David Lecchini (PSL University, French Polynesia), and Frederic Bertucci (University of Montpelier, France). The paper was published in November 2024 in the open access Nature scientific journal NPJ Biodiversity (https://doi.org/10.1038/s44185-024-00064-4).

The study is the first large-scale phylogenetic investigation on fish bioacoustics within this family and examines whether it is feasible to use sounds for taxonomic identification of species. Members of the Holocentridae produce sounds through the “contraction of paired bilateral sonic muscles originating on the skull and inserted on articulated ribs in tight connection with the swim bladder.” These contractions produce vibrations that are amplified by the swim bladder. All 73 species that were investigated had certain characteristics that made this family ideal for this type of study. This is especially important because holocentrids are nocturnal and rely heavily on vocal communication in the dark.

From May 2019 to July 2022, almost 400 specimens were collected from coral reefs of French Polynesia, Guadeloupe, Guam, Philippines and Seychelles. Sounds made by the specimens were recorded while the fish were hand-held in the field, rather than in aquariums, to minimize artifacts (false signals not generated by the fish but rather picked up in the recording process), and provide the most reliable sound comparisons. The sounds that are created vary by species and can range from grunts to clicks to thumping noises. After recording these sounds, the fish were measured and photographed to confirm species identity.

From May 2019 to July 2022, almost 400 specimens were collected from coral reefs of French Polynesia, Guadeloupe, Guam, Philippines and Seychelles. Sounds made by the specimens were recorded while the fish were hand-held in the field, rather than in aquariums, to minimize artifacts (false signals not generated by the fish but rather picked up in the recording process), and provide the most reliable sound comparisons. The sounds that are created vary by species and can range from grunts to clicks to thumping noises. After recording these sounds, the fish were measured and photographed to confirm species identity.

While species-specific sounds of animals have been extensively documented, there is limited phylogenetic research on acoustic behavior of fish species. “It’s possible to suggest that no two species make the same exact sound, even though they’re closely related,” said Donaldson. “They may have components that are the same but there’s something slightly different here or something radically different if you go from one genus to the next.”

This study demonstrates that sounds can indicate different taxonomic groups, although this depends on taxonomic level (i.e. family, genus or species). It was found that the higher the taxonomic level, the easier it was to differentiate them based on sounds.

A significant portion of this collaborative study was supported by Guam NSF EPSCoR thanks to an award from the National Science Foundation’s Established Program to Stimulate Competitive Research (EPSCoR). Donaldson is Guam EPSCoR’s Principal Investigator and Project Director.

Donaldson has also co-authored, with Banse, Parmentier, and others, a publication based on a smaller study that describes acoustic communication between nine species of squirrelfishes in their relationship with cleaner fish. “Production of sounds by squirrelfish during symbiotic relationships with cleaner wrasses,” published in Scientific Reports in May 2024 (https://doi.org/10.1038/s41598-024-61990-8), describes the ability of squirrelfish to communicate their desire to be cleaned by cleaner wrasses through the use of acoustic signals. Scientific Reports is also an open access journal from Nature that publishes original research in the areas of natural science, psychology, medicine, and engineering.

Terry
Terry Donaldson, Ph.D., recently co-authored a paper on bioacoustic communication within the family Holocentridae, a group of marine fishes commonly known as squirrelfishes and soldierfishes. These fishes are known to communicate by sound production. The story is titled, “Sounds as taxonomic indicators in holocentrid fishes,” and was published at the end of 2024. Photo courtesy of the University of Guam.

Graduate research assistants continue to make impact

Grace McDermott displays coral samples at the Mote Marine Lab in Sarasota, Florida. Photo courtesy of Grace McDermott.

EPSCoR 241118 GraceMcDermott 1Grace McDermott displays coral samples at the Mote Marine Lab in Sarasota, Florida. Photo courtesy of Grace McDermott.

As the year 2024 reaches its end, four former Guam NSF EPSCoR Graduate Research Assistants (GRA) share where they are now, and how their time at the University of Guam helped them get there.

The Guam NSF EPSCoR Graduate Research Assistantship (GRA) is a 3-year program that allows graduate students with a Bachelor of Science in Biology or related studies the chance to fully immerse themselves in their fields. Within the program, GRA student researchers can engage in fieldwork and utilize various oceanographic research instruments to investigate coral reefs and further develop research for their theses.

Carlos Tramonte studied under Bastian Bentlage, PhD, from 2020 to 2023, focusing on coral species replacements on the island’s reef flats. More specifically, Tramonte spent much of his time investigating coral-associated Symbiodiniaceae and their response to environmental stressors. Currently residing in Oahu, Hawaii, Tramonte is in his second year of the University of Hawaii’s Institute of Marine Biology PhD program.

EPSCoR 241031 CarlosTramonte 2

 

Carlos Tramonte conducting coral reef field work in Oahu, Hawaii. Photo courtesy of Carlos Tramonte.

“My time at UOG laid a lot of the groundwork for everything I do today. The skills I developed in project design, data analysis, and environmental science, along with my first real experience in Pacific reef ecosystems as a graduate student, have been essential”, says Tramonte. “UOG’s programs helped solidify my commitment to Pacific conservation and gave me the tools to pursue complex projects, such as this current research on reef biodiversity.”

Now an official member of the Guam NSF EPSCoR Biodepository team, Kelsie Ebeling-Whited, initially did research on the spawning of sixbar wrasse fish under project director Terry Donaldson, PhD from 2017 to 2020. 

Regarding her academic stint in the EPSCoR Biodepository, Whited describes that it “helped me discover my interest in historical collections and museum work.” Her transition to a full-time role with the team allowed her to continue the work she started as a GRA student researcher.

Therese Miller also studied under Bentlage for her 2020-2023 GRA experience, in which she investigated bacterial communities living within staghorn coral, most specifically Acropora pulchra. She also studied communities of bacteria living in Porites cylindrica and Porites lobata as part of an Guam NSF EPSCoR-funded project, which included transplantation experiments and coral monitoring/sampling. 

Miller now resides in Nelson, New Zealand and is working towards her PhD in Marine Science through the University of Auckland. Her current research involves following migration routes of two freshwater eel species native to New Zealand: Anguilla dieffenbachii and Anguilla australis schmidtii.

EPSCoR 241203 ThereseMiller

 

Therese Miller (front middle) and her colleagues at sea in New Zealand. Photo courtesy of Therese Miller.

Miller had just recently completed a five-week long research voyage in her and her peers collected seawater samples from Wellington to New Caledonia. 

“I’m about to start lab-work with the samples I collected from my time at sea, and the molecular biology I learned at UOG has given me plenty of experience for the amount of work I am now expected to do,” Miller explains. 

“I also learned other valuable skills during my master’s, such as how to conduct literature reviews, how to organize and prepare my samples, how to work on a team of other researchers, and what the structure of a research-based degree is like.”

Under the guidance of UOG Marine Lab Director Laurie Raymundo, PhD, Grace McDermott studied the phenotypic differences in coral during her GRA program ending in 2023. These days, she works as a biologist and laboratory manager at the Mote Marine Laboratory in Sarasota, Florida, while also preparing to apply for PhD programs. 

Her current research includes running experiments to identify coral species candidates for Mote’s coral restoration and reproduction programs, as well as studying situ coral disease dynamics and novel antibiotic-free treatment efficacy.

EPSCoR 241118 GraceMcDermott 2

 

Grace McDermott poses for a selfie in the lab at Mote Marine Lab, Florida. Photo courtesy of Grace McDermott.

“My time at the UOG Marine Lab helped me cultivate experience organizing and leading experiments in the field and in the lab testing the resilience of corals to stress,” McDermott details. “The accessibility of Guam’s coral reefs provided me with the opportunity to explore my interest in the impacts of future climate change scenarios on marine ecosystems.”

Grace McDermott displays coral samples at the Mote Marine Lab in Sarasota, Florida. Photo courtesy of Grace McDermott.
Grace McDermott, 29, displaying coral samples at the Mote Marine Lab in Sarasota, Florida.

As the year 2024 reaches its end, four former Guam NSF EPSCoR graduate research assistants share where they are now, and how their time at the University of Guam helped them get there.

The Guam NSF EPSCoR Graduate Research Assistantship (GRA) is a 3-year program that allows graduate students with a Bachelor of Science in Biology or related studies the chance to fully immerse themselves in their fields. Within the program, GRA student researchers can engage in fieldwork and utilize various oceanographic research instruments to investigate coral reefs and further develop research for their theses.

Carlos Tramonte studied under Bastian Bentlage, PhD, from 2020 to 2023, focusing on coral species replacements on the island’s reef flats. More specifically, Tramonte spent much of his time investigating coral-associated Symbiodiniaceae and their response to environmental stressors. Currently residing in Oahu, Hawaii, Tramonte is in his second year of the University of Hawaii’s Institute of Marine Biology PhD program.

Carlos Tramonte conducting coral reef field work in Oahu, Hawaii. Photo courtesy of Carlos Tramonte.
Carlos Tramonte conducting coral reef field work in Oahu, Hawaii. Photo courtesy of Carlos Tramonte.

“My time at UOG laid a lot of the groundwork for everything I do today. The skills I developed in project design, data analysis, and environmental science, along with my first real experience in Pacific reef ecosystems as a graduate student, have been essential”, says Tramonte. “UOG’s programs helped solidify my commitment to Pacific conservation and gave me the tools to pursue complex projects, such as this current research on reef biodiversity.”

Now an official member of the Guam NSF EPSCoR Biorepository team, Kelsie Ebeling-Whited, initially did research on the spawning of sixbar wrasse fish under project director Terry Donaldson, PhD from 2017 to 2020. 

Regarding her academic stint in the Guam NSF EPSCoR Biorepository, Whited describes that it “helped me discover my interest in historical collections and museum work.” Her transition to a full-time role with the team allowed her to continue the work she started as a GRA student researcher.

Therese Miller also studied under Bentlage for her 2020-2023 GRA experience, in which she investigated bacterial communities living within staghorn coral, most specifically Acropora pulchra. She also studied communities of bacteria living in Porites cylindrica and Porites lobata as part of an Guam NSF EPSCoR-funded project, which included transplantation experiments and coral monitoring/sampling. 

Miller now resides in Nelson, New Zealand and is working towards her PhD in Marine Science through the University of Auckland. Her current research involves following migration routes of two freshwater eel species native to New Zealand: Anguilla dieffenbachii and Anguilla australis schmidtii.

Therese Miller (front middle) and her colleagues at sea in New Zealand. Photo courtesy of Therese Miller.
Therese Miller (front middle) and her colleagues at sea in New Zealand. Photo courtesy of Therese Miller.

Miller had just recently completed a five-week long research voyage in her and her peers collected seawater samples from Wellington to New Caledonia. 

“I’m about to start lab-work with the samples I collected from my time at sea, and the molecular biology I learned at UOG has given me plenty of experience for the amount of work I am now expected to do,” Miller explains. 

“I also learned other valuable skills during my master’s, such as how to conduct literature reviews, how to organize and prepare my samples, how to work on a team of other researchers, and what the structure of a research-based degree is like.”

Under the guidance of UOG Marine Lab Director Laurie Raymundo, PhD, Grace McDermott studied the phenotypic differences in coral during her GRA program ending in 2023. These days, she works as a biologist and laboratory manager at the Mote Marine Laboratory in Sarasota, Florida, while also preparing to apply for PhD programs. 

 

Grace McDermott poses for a selfie in the lab at Mote Marine Lab, Florida. Photo courtesy of Grace McDermott.
Grace McDermott poses for a selfie in the lab at Mote Marine Lab, Florida. Photo courtesy of Grace McDermott.

Her current research includes running experiments to identify coral species candidates for Mote’s coral restoration and reproduction programs, as well as studying situ coral disease dynamics and novel antibiotic-free treatment efficacy.

“My time at the UOG Marine Lab helped me cultivate experience organizing and leading experiments in the field and in the lab testing the resilience of corals to stress,” McDermott details. “The accessibility of Guam’s coral reefs provided me with the opportunity to explore my interest in the impacts of future climate change scenarios on marine ecosystems.”

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