Research - Guam EPSCoR

December 26, 2023January 13, 2026

University of Guam researchers develop new way of analyzing coral-associated microalgae

UOG biology alumnus Colin Anthony authored the research report “Rapid, high-throughput phenotypic profiling of endosymbiotic dinoflagellates (Symbiodiniaceae) using benchtop flow cytometry,” which was published in PLoS ONE in September 2023 (Photos courtesy of Guam NSF EPSCoR).

UOG biology alumnus Colin Lock co-authored the paper along with Anthony and assisted with the development of the new method (Photos courtesy of Guam NSF EPSCoR).

This new method of analyzing Symbiodiniaceae utilizes flow cytometry, a technique that uses lasers to detect and measure the physical and chemical characteristics of a population of cells (Photos courtesy of Guam NSF EPSCoR).

Before a sample can be processed by a flow cytometer, algal cells have to be separated from the coral skeleton and coral tissue (Photos courtesy of Guam NSF EPSCoR).

Researchers from the University of Guam have published a study in the journal PLoS ONE detailing a new method for analyzing Symbiodiniaceae, a type of microalgae vital to coral reef health. According to the paper, published in September, this procedure allows scientists to receive data within one day compared to other methods that are either expensive, take months to process, or lack resolution.

Symbiodiniaceae is a family of marine microalgae known for their symbiotic relationships with reef-building corals, giant clams, jellyfish, and other marine invertebrates. Understanding Symbiodiniaceae is crucial to those working to protect and restore coral reefs that are under threat around the world due to climate change.

According to the article, many techniques used to analyze Symbiodiniaceae are often low resolution, expensive, and inaccessible to researchers.

To address this issue, two research associates from the UOG Marine Laboratory developed a protocol that collects information on cell shape, size, and photopigments for thousands of Symbiodiniaceae cells in just a few minutes.

“We basically showed that you can use flow cytometry to better understand coral-associated algae,” said lead author Colin Anthony, a former Guam NSF EPSCoR graduate research assistant. “Using this method, you remove the algal cells from within the coral tissue, process them with the flow cytometer which hits them with a bunch of lasers, and then you get an idea of the state of the algae and its ability to acclimate or adapt to environmental conditions.”

Flow cytometry is a technique that uses lasers to detect and measure the physical and chemical characteristics of a population of cells. This technology is typically used in drug testing and cancer research.

“It’s a pretty widely accepted technique,” said co-author Colin Lock. “Within the coral world, flow cytometry is hugely underutilized. Regarding algae, what we’re finding out is that there are different species with different tolerances, so you can look at the shifts between species within a coral host.”

Anthony developed this new method while pursuing his graduate degree at UOG.

“When I started my master’s in 2020, I thought I was going to look at the biodiversity of Symbiodiniaceae,” said Anthony. “I later realized that there were all these other aspects of flow cytometry that people don’t really acknowledge or understand in the coral community.”

While the technique mentioned in the paper is designed for corals, Anthony said that with slight modification, it can be used for any organism that contains endosymbiotic algae such as upside-down jellyfish as well as any free-living ocean algae.

Because this method produces such a high output of data in a significantly shorter amount of time compared to other procedures, both Anthony and Lock hope that other scientists within their field start using this technique.

“It’s a lot of work to come up with a new method because there’s so much troubleshooting involved with its development,” said Lock. “This can be used to examine responses in a lot of different organisms, and we just hope that people adopt it and start integrating it into their work.”

December 26, 2023January 13, 2026

Guam NSF EPScoR installs new Micro-CT scanner

Diego Vaz, Ph.D., associate curator of the GECCO Biorepository, sits next to the new Micro-CT scanner installed by Guam NSF EPSCoR. The scanner represents a significant increase to UOG’s research capacity as it is now one of the few educational institutions within the Asia-Pacific region equipped with an instrument of this caliber (Photos courtesy of Guam NSF EPSCoR).

Scan of a fish skull taken with the new Micro-CT scanner. The scanner will be used for projects for the GECCO Biorepository, a physical and cyber natural history collection operated by Guam NSF EPSCoR that holds records and images of marine organisms found throughout the Pacific and other locales (Photos courtesy of Guam NSF EPSCoR).

Guam NSF EPSCoR has installed a new Micro-Computer Tomography (CT) scanner at the University of Guam—making the university one of the few educational institutions within the Asia-Pacific region equipped with an instrument of this caliber.

The acquisition of this instrument was made possible by the support of the U.S. National Science Foundation’s (NSF) Established Program to Stimulate Competitive Research (EPSCoR) under Grant Number OIA-1946352.

Similar to those used in hospitals, a Micro-CT scanner offers a non-invasive way of studying organisms by taking X-ray images of a specimen from various angles and combining them to create three-dimensional images.

The scanner, which was installed in August 2023, will be used for projects associated with the Guam Ecosystems Collaboratorium for Corals and Oceans (GECCO) Biorepository, a physical and cyber natural history collection operated by Guam NSF EPSCoR that holds records and images of marine organisms found throughout the Pacific and other locales.

Using the scanner, researchers can visualize the inner structures of various organisms such as corals, sponges, mollusks, shrimp, or fish. Scans of different specimens can provide a deeper understanding of marine life in the Marianas and the region by offering valuable insight into internal anatomy and how species differ from one another.

“You can couple that information with the information about the ecology of the organism and their genetic relationships. By doing that, you can build a much stronger understanding of the biodiversity in this region,” said Guam NSF EPSCoR Principal Investigator and Project Director Terry Donaldson, Ph.D.

Building capacity

Staff from the GECCO Biorepository trained from Aug. 28 – 30 to familiarize themselves with the scanner. During the sessions, they learned how to prepare specimens for a scan, reconstruct the created files, and process the data.

“One of our goals regarding the scanner is training more people on how to use it. We want to increase capacity and teach students and researchers how to make the most of this machine,” said Diego Vaz, Ph.D., associate curator of the GECCO Biorepository.

Promoting growth

The scanner will enhance collaborative activity, promoting growth in marine taxonomy, ecology, and other fields of research.

“Having the scanner is going to open a lot of doors for what we can do here. We’re excited to have this instrument because it increases our footprint,” said Donaldson. “The installation of the scanner in a facility directly adjacent to a coral reef ecosystem allows for the creation of a Micro-CT Research Node that will draw researchers from all over the region, and indeed, the world, to utilize the instrument in a unique setting. The Marine Laboratory and the University of Guam will become much more visible, and more and more researchers will want to work with us which means collaborations, publications, and the advancement of science.”

August 17, 2023January 13, 2026

UOG grad student talks coral reefs at aquatic sciences conference

University of Guam graduate biology student and Guam NSF EPSCoR Graduate Research Assistant Star Dressler presented her research at the 2023 Association for the Sciences of Limnology and Oceanography (ASLO) Aquatic Sciences Meeting held from June 4 – 9, 2023, in Palma de Mallorca, Spain.

ASLO is an organization that fosters a diverse, international scientific community that creates, integrates, and communicates knowledge across the full spectrum of aquatic sciences.

During the event, Dressler presented her research entitled, “Assessing the heterotrophic abilities of two scleractinian corals during thermal stress as a strategy.”

“ASLO was awesome,” said Dressler. “I got to interact with scientists from all over the world. It was an oceanography and limnology-based conference, which means it covered both marine and freshwater topics. I was able to not only listen to everyone’s discussions about diverse topics, but also bring attention to coral reefs.”

Along with her advisor, UOG Associate Professor of Oceanography Atsushi Fujimura, Dressler also chaired a session called “Resilience in Coral Reef Ecosystems.” The session featured various scientists who discussed advancements in understanding resilience in the world’s coral reef ecosystems across species, population, and community levels.

“I appreciated being able to connect with different scientists and receive a lot of information in such a short time. I would love to continue being a member of ASLO and going to their conferences,” said Dressler.

Star ASLO Photo 2 2 — During the event, Dressler presented her research entitled, “Assessing the heterotrophic abilities of two scleractinian corals during thermal stress as a strategy.” “ASLO was awesome,” said Dressler. “I got to interact with scientists from all over the world. It was an oceanography and limnology-based conference, which means it covered both marine and freshwater topics. I was able to not only listen to everyone’s discussions about diverse topics, but also bring attention to coral reefs.”

July 18, 2023September 6, 2023

UOG researcher explores the biodiversity of crabs in the Red Sea and Persian Gulf

The specimens gathered from these expeditions will be placed in the Guam Ecosystems Collaboratorium for Corals and Oceans (GECCO) Biorepository, a physical and cyber warehouse of records and images operated by the Guam NSF EPSCoR program. Pictured here are specimens gathered from the Red Sea.

UOG Curator of Crustacea Robert Lasley went on two research expeditions to conduct biodiversity surveys within the Red Sea and the Persian Gulf.

With support from the King Abdullah University of Science and Technology in Saudi Arabia, Lasley joined a team of biologists and spent two weeks at sea collecting specimens such as crabs, mollusks, and worms from May 3 to May 27, 2023.

During this expedition, Autonomous Reef Monitoring Structures (ARMS) were deployed. ARMS are stacks of plates that mimic the sea floor. They are used to recruit local species over a set period of time to gauge and compare marine biodiversity among habitats, islands, and even ocean basins. Lasley and his colleagues will eventually deploy ARMS in Guam.

During his time in Abu Dhabi from June 16 to June 29, Lasley explored the Persian Gulf with a team of scientists as part of a trip sponsored by Archireef, a Hong Kong-based company that builds 3D-printed reef structures to restore degraded coral reefs.

The specimens gathered from these expeditions will be placed in the Guam Ecosystems Collaboratorium for Corals and Oceans (GECCO) Biorepository, a physical and cyber warehouse of records and images operated by the Guam NSF EPSCoR program.

“I’m really interested in crab biodiversity and biogeography in general,” said Lasley. “The Indo-West Pacific – which includes Guam, the Red Sea, and the Persian Gulf – is this massive biogeographic region and some species are connected throughout it. Any specimens I collect anywhere are good to bring to our collection for comparative material because as we do research, we need to compare specimens from Guam but also specimens from elsewhere.”

Between the two trips, Lasley collected more than 500 specimens, which include around 100 to 200 species of crabs.

“We have already found a new record and, possibly, a new species from the Red Sea,” Lasley said. “We are bound to find more as I examine the specimens more thoroughly.”

July 3, 2023January 13, 2026

UOG grad student explores the small world of meiofauna

Over the course of the workshop, Vega was able to connect with meiofauna experts from around the world as well as learn different collection and sampling techniques for DNA metabarcoding of meiofauna communities around Dauphin Island.

“Being in the workshop was very hard in the beginning, but I got to learn a lot by asking other people how they’d process their samples so by the end of the workshop, I was faster,” said Vega. “As a scientist, I feel like if there’s any new skill I would like to have, I’ll just have to practice and I’ll get better. Learning is infinite and it’s exciting to know that.”

Sarai Vega, a biology graduate student from the University of Guam was one of 13 participants selected worldwide to attend the 2023 Dauphin Island Sea Lab Meiofauna Diversity and Taxonomy Workshop in Alabama from May 10 to May 19, 2023.

Meiofauna are invertebrates that live in both marine and freshwater environments that are small enough to pass through a 0.45 micrometer mesh. They live in between the grains of sand and mud on the seafloor and riverbeds. Copepods, flatworms, and nematodes fall under meiofauna.

Because the meiofauna group is broad, the field remains understudied despite its ecological importance.

“Meiofauna have a high turnover, so they reproduce very fast and they don’t live very long,” said Vega. “Because of this, they are a food source to bigger animals like crabs and sea cucumbers. In turn, those bigger animals are food for other animals. If this group didn’t exist, it would affect that chain.”

Over the course of the workshop, Vega was able to connect with meiofauna experts from around the world as well as learn different collection and sampling techniques for DNA metabarcoding of meiofauna communities around Dauphin Island.

As a Guam NSF EPSCoR Graduate Research Assistant, Vega became interested in researching meiofauna to incorporate it into her thesis project, which focuses on studying the formation of sediment plumes in the Pago Watershed in central Guam and how they affect the island’s environment.

Sediment from soil erosion due to fires and invasive species such as wild pigs and deer can wash into rivers, streams, and bays during rainfall. Once in the water, these plumes of sediment can pollute bodies of water and smother coral reef systems.

“These sediment plumes consist of very fine sediment,” said Vega. “I want to understand what’s happening and how they affect meiofauna.”

Vega said that her experience attending the workshop has benefited her development as a scientist.

“Being in the workshop was very hard in the beginning, but I got to learn a lot by asking other people how they’d process their samples so by the end of the workshop, I was faster,” said Vega. “As a scientist, I feel like if there’s any new skill I would like to have, I’ll just have to practice and I’ll get better. Learning is infinite and it’s exciting to know that.”

Sarai Vega, a biology graduate student from the University of Guam was one of 13 participants selected worldwide to attend the 2023 Dauphin Island Sea Lab Meiofauna Diversity and Taxonomy Workshop in Alabama from May 10 to May 19, 2023.

Meiofauna are invertebrates that live in both marine and freshwater environments that are small enough to pass through a 0.45 micrometer mesh. They live in between the grains of sand and mud on the seafloor and riverbeds. Copepods, flatworms, and nematodes fall under meiofauna.

May 1, 2023December 16, 2025

Anthony defends his Master of Science in Biology

Guam NSF EPSCoR Graduate Research Assistant Colin Anthony defended his thesis in front of his mentors, classmates and teachers at the University of Guam Marine Lab in Mangilao.

His thesis was titled, “Acclimation of Endosymbiotic Symbiodiniaceae: Improved Insights through Flow Cytometric Phenotypic Profiling.”

His chairperson was Dr. Bastian Bentlage. Colin’s committee consisted of Dr. Brett Taylor from the University of Guam and Dr. Cheryl Ames from Tohoku University.

April 27, 2023December 16, 2025

Guam NSF EPSCoR showcases research at sustainability conference

Graduate students, postdocs, as well as other researchers were able to speak about their work to attendees of the University of Guam Conference on Island Sustainability Conference on April 14, 2023, at the Hyatt Regency Guam.

The presentations were a part of the Guam Ecosystems Collaboratorium for Corals and Oceans, a breakout session presented by Guam NSF EPSCoR. The event was moderated by Sarah Lemer, Ph.D., an assistant professor of marine invertebrate genomics at the University of Guam.

Presenters included Guam NSF EPSCoR Graduate Research Assistants Colin Anthony, Therese Miller, Renee Crisostomo, and Joseph Proietti. They covered a range of topics such as using publicly available data to study global jellyfish distribution as well as evolutionary trends, the microbiome of the staghorn coral Acropora pulchra from West Hagåtña Bay, Phenotypic plasticity in Acropora aspera and its implications for coral restoration, and quantifying genotypic diversity in the coral Porites rus.

Marilyn Brandt, Ph.D., a research associate from the Center for Marine and Environmental Studies at the University of the Virgin Islands talked about Rescue to Reef, a program that links science-based coral restoration to privately-owned resorts within the U.S. Virgin Islands.

Other presenters at the breakout session included postdocs Hector Torrado and Gaurav Shimpi, who discussed their research regarding the relatedness and clonality of Acropora corals on Guam as well as mitochondria and soft corals.

Lastly, David Burdick, who manages Guam’s long-term reef monitoring program, talked about how the island’s coral reefs have changed over the last decade and how different parts of the reefs respond differently to stressors.

April 24, 2023December 16, 2025

JOIN US: Colin J Anthony to defend his Master of Science: Biology thesis!

Join us on Friday, April 28th, 2023 at 10:00 AM ChST as we cheer on our student researcher Colin J Anthony as he defends his Master of Science: Biology thesis!

Thesis Title: Acclimation of Endosymbiotic Symbiodiniaceae: Improved Insights through Flow Cytometric Phenotypic Profiling

Chairperson: Dr. Bastian Bentlage (Committee: Dr. Brett Taylor & Dr. Cheryl Ames (Tohoku University)

Location: Marine Laboratory Classroom Room 205

Zoom link: http://ow.ly/6XxQ50NQ4zP

March 16, 2023December 16, 2025

Visiting scholar talks nurseryfish, megamouth shark research at UOG Marine Laboratory

For the month of February, Guam NSF EPSCoR welcomed Tim M. Berra, Ph.D., a professor emeritus and academy professor at the Ohio State University as a visiting NSF EPSCoR scholar.

Berra is a three-time recipient of Fulbright Fellowships to Australia and has authored over 85 scientific papers and 9 books including Freshwater Fish Distribution and A Natural History of Australia.

In 2001, Berra began a long-term field project studying the life history of nurseryfish (Kurtus gulliveri).

Nurseryfish live in the fresh and brackish waters of Papua New Guinea as well as parts of northern Australia. Males of this species carry the egg cluster on a hook that protrudes over their forehead.

This trip was Berra’s first-time exploring Micronesia and his experiences on Guam will help contribute to a book he is writing about the Pacific.

“One of my primary interests in being here is that I’m working on a book about the peopling of the Pacific,” said Berra. “I’ve been throughout Polynesia, Melanesia, and now Micronesia. I wanted to find some answers. Where did these people come from? How long ago was it? How did they get here and what did they bring with them?”

For his research, Berra connected with several local experts such as Michael Carson, Ph.D., a University of Guam Associate Professor of Archaeology.

During his time on Guam, Berra gave three presentations to UOG Marine Laboratory students and faculty regarding his work studying nurseryfish in Australia, the challenges associated with preserving a rare 15-foot megamouth shark, and the descendants of Charles Darwin.

Regarding his time spent at the UOG Marine Laboratory, Berra expressed his excitement about the research being conducted at the facility.

“I’ve met so many people who are dealing with so many important topics like coral bleaching and restoration as well as how climate change affects marine ecosystems,” said Berra. “This is a lively place and students have such a great opportunity when it comes to the Marine Laboratory and EPSCoR to start their careers and make a contribution to science and society.”

January 31, 2023December 16, 2025

Graduate student presents at American Society of Naturalists Conference

Kenzie Pollard, a University of Guam graduate biology student and Guam NSF EPSCoR Graduate Research Assistant, presented her research at the 2023 American Society of Naturalists Conference which was held from Jan. 6 – 10, 2023, in Pacific Grove, California.

The American Society of Naturalists is the oldest scientific society dedicated to the study of ecology, evolution, and behavior. The event was fully in-person and included researchers from physiology, phylogenetics, genetics, and other associated fields.

This year’s conference focused on what it means to be a naturalist and researcher in the 21st century.

During the event, Pollard presented her project, entitled, “Cryptic diversity and population connectivity of the coral guard crab, Trapezia bidentata.”

“It was my first time presenting a talk at an international conference and while I was nervous, it was exciting to share what I had spent the last few years on,” said Pollard. “I even had a professor from the University of Florida reach out to me to discuss my research and our shared interest in pocilloporid corals.”

According to Pollard, she appreciated being able to attend the talks held at the event.

“The conference itself was intriguing and packed full of interesting talks,” said Pollard. “The most impactful was the symposium on “Confronting the Legacy of Eugenics in EEB.” A necessary conversation, it raised the voices of underrepresented groups in STEM and focused on the history and impacts of eugenics as well as emphasizing what actions we may take to prevent the perpetuation of these ideologies.”

During the conference, Pollard was able to make new connections easily.

“I could meet somebody new at every meal, and coffee breaks between sessions were great opportunities to approach speakers and chat about their research,” said Pollard. “I was fortunate to attend the conference with colleagues from my undergrad and a prospective advisor for my Ph.D. They introduced me to several scientists in their network and it truly helped build my community.”